Dlx gene expression during chick inner ear development
Dlx gene expression during chick inner ear development
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DOI：
10.1002/cne.20418
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发表时间：
2005-02-28
影响因子：
2.5
通讯作者：
Groves, AK
中科院分区：
文献类型：
--
作者：
Brown, ST;Wang, JM;Groves, AK
Dlx基因家族成员在斑马鱼和小鼠内耳的发育中起重要作用，但Dlx基因与鸟类内耳发育的关系却知之甚少。我们研究了Dlx 1，Dlx 2，Dlx 3，Dlx 5和Dlx 6在鸡胚胎发育的前7天的内耳表达模式。Dlx 1和Dlx 2的表达仅见于21期至32期的耳蜗前庭神经节和神经的非神经元细胞中。Dlx 3标记在第9阶段开始的耳基板，并且随着基板的内陷开始而变得限于与后脑相邻的上皮。然后，DLX 3表达在背侧耳囊肿中消失，并逐渐限于内淋巴囊，到第30期。Dlx 5和Dlx 6在发育中的内耳中的表达分别在第12和13阶段首次见于耳窝的边缘，然后扩散到整个背侧耳囊。随着形态发生的进行，Dlx 5和Dlx 6的表达在整个形成的半规管和内淋巴结构中可见。在后期阶段，这两个基因被认为是标记的远端表面的形成运河和显示的表达互补的BMP 4在前庭感觉区。到第30期，在壶腹斑和耳蜗及前庭神经中也观察到Dlx 5表达。这些发现表明Dlx基因在鸟类内耳的前庭和神经发育中发挥重要作用。(C)2005 Wiley-Liss，Inc.
Members of the Dlx gene family play essential roles in the development of the zebrafish and mouse inner ear, but little is known regarding Dlx genes and avian inner ear development. We have examined the inner ear expression patterns of Dlx1, Dlx2, Dlx3, Dlx5, and Dlx6 during the first 7 days of chicken embryonic development. Dlx1 and Dlx2 expression was seen only in nonneuronal cells of the cochleovestibular ganglion and nerves from stage 21 to stage 32. Dlx3 marks the otic placode beginning at stage 9 and becomes limited to epithelium adjacent to the hindbrain as invagination of the placode begins. Dlx3 expression then resolves to the dorsal otocyst and gradually becomes limited to the endolymphatic sac by stage 30. Dlx5 and Dlx6 expression in the developing inner ear is first seen at stages 12 and 13, respectively, in the rim of the otic pit, before spreading throughout the dorsal otocyst. As morphogenesis proceeds, Dlx5 and Dlx6 expression is seen throughout the forming semicircular canals and endolymphatic structures. During later stages, both genes are seen to mark the distal surface of the forming canals and display expression complementary to that of BMP4 in the vestibular sensory regions. Dlx5 expression is also seen in the lagena macula and the cochlear and vestibular nerves by stage 30. These findings suggest important roles for Dlx genes in the vestibular and neural development of the avian inner ear. (C) 2005 Wiley-Liss, Inc.