A wide-range phylogenetic analysis of Zic proteins: Implications for correlations between protein structure conservation and body plan complexity
A wide-range phylogenetic analysis of Zic proteins: Implications for correlations between protein structure conservation and body plan complexity
复制标题
DOI：
10.1016/j.ygeno.2006.02.011
复制
发表时间：
2006-06-01
期刊：
影响因子：
4.4
通讯作者：
Toyoda, Atsushi
中科院分区：
文献类型：
--
作者：
Aruga, Jun;Kamiya, Akiko;Toyoda, Atsushi
我们比较了来自多种动物的 Zic 同源物。在锌指结构域中发现了惊人的保守性，其中所有双边动物中都保留了外显子-内含子边界，但enidarians中没有，这表明所有双边Zic基因都源自双边祖先中的单个基因。还有额外的保守氨基酸序列 ZOC 和 ZF-NC。根据蛋白质结构保守程度，对锌指、ZOC 和 ZF-NC 的联合分析揭示了两类 Zic 的存在。 “保守”类别包括来自节肢动物、软体动物、环节动物、棘皮动物和脊索动物（脊椎动物和头索动物）的 Zic 蛋白，而“分化”类别则包括来自扁形动物、刺胞动物、线虫动物和脊索动物（尾索动物）的 Zic 蛋白。结果表明，祖先双侧 Zic 蛋白已经获得了一整套保守结构域，但在扁形动物、线虫和尾索动物中却丢失并出现了分歧。 (c) 2006 Elsevier Inc. 保留所有权利。
We compared Zic homologues from a wide range of animals. Striking conservation was found in the zinc finger domains, in which an exon-intron boundary has been kept in all bilateralians but not enidarians, suggesting that all of the bilateralian Zic genes are derived from a single gene in a bilateralian ancestor. There were additional conserved amino acid sequences, ZOC and ZF-NC. Combined analysis of the zinc finger, ZOC, and ZF-NC revealed the presence of two classes of Zic, based on the degree of protein structure conservation. The "conserved" class includes Zic proteins from the Arthropoda, Mollusca, Annelida, Echinodermata, and Chordata (vertebrates and cephalochordates), whereas the "diverged" class contains those from the Platyhelminthes, Cnidaria, Nematoda, and Chordata (urochordates). The result indicates that the ancestral bilateralian Zic protein had already acquired an entire set of conserved domains, but that this was lost and diverged in the platyhelminthes, nematodes, and urochordates. (c) 2006 Elsevier Inc. All rights reserved.