Radioimmunological detection of anti-transglutaminase autoantibodies in human saliva: a useful test to monitor coeliac disease follow-up
Radioimmunological detection of anti-transglutaminase autoantibodies in human saliva: a useful test to monitor coeliac disease follow-up
复制标题
DOI：
10.1111/j.1365-2036.2008.03720.x
复制
发表时间：
2008-08-01
影响因子：
7.6
通讯作者：
Tiberti, C.
中科院分区：
文献类型：
--
作者：
Bonamico, M.;Nenna, R.;Tiberti, C.
背景血清放射免疫测定（RIA）组织转谷氨酰胺酶自身抗体（tTG-Abs）被证明在乳糜泻（CD）随访期间也是一种敏感的测试。我们证明可以在人类唾液中检测到 RIA tTG-Abs。目的评估采用无麸质饮食 (GFD) 的乳糜泻儿童的唾液 RIA tTG-Abs。方法在诊断时对 109 名乳糜泻儿童的唾液和血清样本进行评估（第 1 组：71 名女性，中位年龄 9.4 岁），其中 58 名接受 GFD 治疗的儿童：3-6 个月后 36 名（组2a)，9 个月或以上时 34 名（组 2b）。两个性别和年龄匹配的对照组：89 名胃肠病患者（第 3 组）和 49 名健康受试者（第 4 组）参与了该研究。采用RIA法检测唾液和血清tTG-Abs，并与血清tTG-Abs ELISA和IgA抗肌内膜抗体（EMA）进行比较。结果唾液RIA tTG-Abs在1、2a和2b组CD患者中检出率为94.5%、66.7%和50.0%，在CD患者中检出率为98.2%、72.2%和98.2%。分别为相应血清样本的50.0%。 tTG-Abs 随着 GFD 进展而降低，并且唾液滴度和血清滴度之间存在相关性（r = 0.75，P = 0.0001）。在 CD 随访期间，唾液和血清 RIA 敏感性相当，并且相对于 EMA 和 ELISA 更高。结论本研究表明，不仅在 CD 诊断时而且在 GFD 期间都可以高灵敏度检测唾液 tTG-Ab。
BackgroundSerum radioimmunoassay (RIA) tissue transglutaminase autoantibodies (tTG-Abs) proved to be a sensitive test also during coeliac disease (CD) follow-up. We demonstrated that RIA tTG-Abs could be detected in human saliva.AimTo evaluate salivary RIA tTG-Abs in coeliac children on gluten-free diet (GFD).MethodsSaliva and serum samples from 109 coeliac children were evaluated at diagnosis (group 1: 71 females, median age 9.4 years) and 58 of them on GFD: 36 after 3-6 months (group 2a), 34 at 9 months or more (group 2b). Two gender- and age-matched control groups: 89 gastroenterological patients (group 3) and 49 healthy subjects (group 4) participated in the study. Saliva and serum tTG-Abs were detected by RIA and compared with serum tTG-Abs ELISA and IgA anti-endomysium antibodies (EMA).ResultsSalivary RIA tTG-Abs were found in 94.5%, 66.7% and 50.0% of groups 1, 2a and 2b CD patients and in 98.2%, 72.2% and 50.0% of corresponding serum samples, respectively. tTG-Abs decreased with GFD progression and a correlation was found between saliva and serum titres (r = 0.75, P = 0.0001). During the CD follow-up, salivary and serum RIA sensitivities were comparable, and higher with respect to EMA and ELISA.ConclusionsThis study demonstrates that it is possible to detect salivary tTG-Abs with high sensitivity not only at CD diagnosis, but also during GFD.