Muscarinic M2-receptors in rat thoracic dorsal root ganglia
Muscarinic M2-receptors in rat thoracic dorsal root ganglia
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DOI：
10.1016/s0304-3940(99)00300-6
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发表时间：
1999-05-14
影响因子：
2.5
通讯作者：
Kummer, W
中科院分区：
文献类型：
--
作者：
Haberberger, R;Henrich, M;Kummer, W
研究了大鼠胸背根神经节 (DRG) 中毒蕈碱 M2 受体亚型 (M2R) 的发生和分布。通过 RT-PCR 在 DRG 的总 RNA 中证明了 M2R 的信使 RNA。 MPR蛋白的免疫反应性集中于26%的感觉神经元，其中大多数（85%）属于直径25-40μm的大小类别。双标记（免疫）组织化学显示，所有 M2R 免疫反应性神经元均结合凝集素 I-B4，而它们通常缺乏 P 物质免疫反应性。这些数据显示 M2R 存在于可能是伤害性初级传入神经元的亚群上，从而扩展了先前的药理学和电生理学研究，这些研究表明 M2R 和/或 M4R 在抑制大鼠感觉神经元钙通道电流中的作用（Wanke, E.、Bianchi, L.、Mantegazza, M.、Guatteo, E.、Macinelli, E. 和 Ferroni, A.，Ca2+ 的毒蕈碱调节）大鼠感觉神经元中的电流：通道和受体类型、剂量反应关系和串扰通路，(C) 1999 Elsevier Science Ireland Ltd。保留所有权利。
The occurrence and distribution of the muscarinic M2-receptor subtype (M2R) was investigated in rat thoracic dorsal root ganglia (DRG). Messenger RNA for M2R was demonstrated by RT-PCR in total RNA from DRG. Immunoreactivity to MPR-protein was localized to 26% of sensory neurons, the majority of them (85%) belonging to the size class of 25-40 mu m in diameter. Double-labeling (immuno)histochemistry revealed that all M2R-immunoreactive neurons bind the lectin, I-B4, whereas they are generally devoid of substance P-immunoreactivity. These data show the presence of M2R on a subpopulation of presumably nociceptive primary afferent neurons, thereby extending previous pharmacological and electrophysiological studies that indicated a role of M2R and/or M4R in inhibition of calcium channel currents in rat sensory neurons (Wanke, E., Bianchi, L., Mantegazza, M., Guatteo, E., Macinelli, E. and Ferroni, A., Muscarinic regulation of Ca2+ currents in rat sensory neurons: channel and receptor types, dose-response relationships and cross-talk pathways, (C) 1999 Elsevier Science Ireland Ltd. All rights reserved.