Activation of cardiac progenitor cells through paracrine effects of mesenchymal stem cells
Activation of cardiac progenitor cells through paracrine effects of mesenchymal stem cells
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DOI：
10.1016/j.bbrc.2008.06.074
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发表时间：
2008-09-12
影响因子：
3.1
通讯作者：
Nagaya, Noritoshi
中科院分区：
文献类型：
--
作者：
Nakanishi, Chiaki;Yamagishi, Masakazu;Nagaya, Noritoshi
骨髓间充质干细胞（MSC）移植已被证明是治疗心力衰竭的一种有前途的策略。MSC移植的作用被认为主要以旁分泌方式介导。最近的报告表明，心脏祖细胞（CPC）驻留在心脏。在本研究中，我们探讨了MSC是否有旁分泌作用的CPC在体外。采用组织块法从新生大鼠心脏分离CPC。从成年大鼠骨髓中分离MSC。MSC条件培养液促进CPC增殖，抑制缺氧和血清饥饿诱导的CPC凋亡。趋化室实验表明，MSC衍生的条件培养基增强CPC的迁移。此外，MSC衍生的条件培养基上调CPC中心肌细胞相关基因的表达，如β-肌球蛋白重链（β-MHC）和心房利钠肽（ANP）。结论：MSC条件培养液对CPC具有保护作用，促进CPC的迁移和分化。(C)2008年爱思唯尔公司All rights reserved.
Mesenchymal stem cells (MSC) transplantation has been proved to be promising strategy to treat the failing heart. The effect of MSC transplantation is thought to be mediated mainly in a paracrine manner. Recent reports have suggested that cardiac progenitor cells (CPC) reside in the heart. In this study, we investigated whether MSC had paracrine effects oil CPC in vitro. CPC were isolated from the neonatal rat heart using an explant method. MSC were isolated from the adult rat bone marrow. MSC-derived conditioned medium promoted proliferation of CPC and inhibited apoptosis of CPC induced by hypoxia and serum starvation. Chemotaxis chamber assay demonstrated that MSC-derived conditioned medium enhanced migration of CPC. Furthermore, MSC-derived conditioned medium upregulated expression of cardiomyocyte-related genes in CPC such as beta-myosin heavy chain (beta-MHC) and atrial natriuretic peptide (ANP). In conclusion, MSC-derived conditioned medium had protective effects on CPC and enhanced their migration and differentiation. (C) 2008 Elsevier Inc. All rights reserved.