A transfected human muscle cell line expressing the adult subtype of the human muscle acetylcholine receptor for diagnostic assays in myasthenia gravis
A transfected human muscle cell line expressing the adult subtype of the human muscle acetylcholine receptor for diagnostic assays in myasthenia gravis
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DOI：
10.1212/wnl.47.6.1552
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发表时间：
1996-12-01
期刊：
影响因子：
9.9
通讯作者：
Vincent, A
中科院分区：
文献类型：
--
作者：
Beeson, D;Jacobson, L;Vincent, A
将人乙酰胆碱受体(AChR)免疫沉淀法用于重症肌无力(MG)的诊断。我们比较了来自表达胎儿型AChR的TE671细胞的人AChR和我们设计的表达成人型AChR的TE671-epsilon细胞的AChR。一些低滴度的MG血清在这两个亚型之间有明显的区别。在标准检测中有可疑滴度的7份MG血清中，有4份与TE671-epsilon AChR呈阳性滴度，而与TE671细胞呈阳性滴度的只有7份血清中的1份。新的细胞系提供了比从正常人类肌肉中获得的更高浓度的成年AChR，并提高了诊断分析的灵敏度。
Immunoprecipitation of human acetylcholine receptor (AChR) is used in the diagnostic assay for myasthenia gravis (MG). We compared human AChR derived from TE671 cells, which express fetal-type AChR, with AChR from TE671-epsilon cells, which we have engineered to express adult-type AChR. Some low-titer MG sera distinguished strikingly between the two subtypes. Four out of seven MG sera that had equivocal titers in standard assays gave positive titers with TE671-epsilon AChR, whereas only one out of seven gave a positive titer with TE671 cells. The new cell line provides a greater concentration of adult AChR than can be obtained from normal human muscle and increases the sensitivity of the diagnostic assay.