KRAS CODON-12 MUTATIONS OCCUR VERY FREQUENTLY IN PANCREATIC ADENOCARCINOMAS
KRAS CODON-12 MUTATIONS OCCUR VERY FREQUENTLY IN PANCREATIC ADENOCARCINOMAS
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DOI：
10.1093/nar/16.16.7773
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发表时间：
1988-08-25
影响因子：
14.9
通讯作者：
BOS, JL
中科院分区：
文献类型：
--
作者：
SMIT, VTHBM;BOOT, AJM;BOS, JL
分析来自人胰腺癌的DNAs中NRAS、KRAS和HRAS基因的密码子12、13和61中突变的存在。福尔马林固定和石蜡包埋的组织直接用于体外扩增反应以扩增相关RAS序列。使用突变特异性合成寡核苷酸通过选择性杂交检测突变。在30名患者中，我们发现28名患者的KRAS基因第12密码子发生突变。这一结果证实了Almoguera等人[Cell 53（1988）549-554]的发现，即KRAS突变经常发生在外分泌胰腺的腺癌中。这些突变主要是G-T颠换，而结肠肿瘤中的KRAS突变主要是G-A转换。此外，在一部分肿瘤中，突变似乎是纯合的。
DNAs from human pancreatic adenocarcinomas were analyzed for the presence of mutations in codons 12, 13 and 61 of the NRAS, KRAS and HRAS gene. Formalin-fixed and paraffin-embedded tissue was used directly in an in vitro amplification reaction to expand the relevant RAS sequences. The mutations were detected by selective hybridization using mutation-specific synthetic oligonucleotides. In 28 of the 30 patients we found a mutation in codon 12 of the KRAS gene. This result confirms the findings of Almoguera et al. [Cell53(1988) 549–554] that KRAS mutations occur frequently in adenocarcinomas of the exocrine pancreas. The mutations are predominantly G-T transversions, in contrast to the KRAS mutations in colon tumors which are mainly G-A transitions. Furthermore, in a portion of the tumors the mutation appears to be homozygous.