Effect of thyroid hormone‐disrupting chemicals on swim bladder inflation and thyroid hormone‐related gene expression in Japanese medaka and zebrafish
Effect of thyroid hormone‐disrupting chemicals on swim bladder inflation and thyroid hormone‐related gene expression in Japanese medaka and zebrafish
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DOI：
10.1002/jat.4302
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发表时间：
2022-02
影响因子：
3.3
通讯作者：
Y. Horie;Miho Nomura;Konori Okamoto;Chiho Takahashi;Tomomi Sato;S. Miyagawa;H. Okamura;T. Iguchi
中科院分区：
文献类型：
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作者：
Y. Horie;Miho Nomura;Konori Okamoto;Chiho Takahashi;Tomomi Sato;S. Miyagawa;H. Okamura;T. Iguchi
我们比较了甲状腺激素干扰化学物质（七氟丁酸、PFBA和三[1，3-二氯-2-丙基]磷酸盐、TDCPP）和甲状腺激素（3，3 '，5-三碘-L-甲状腺原氨酸、T3）对日本青鳉鱼和斑马鱼鱼鳔充气和甲状腺激素相关基因表达的影响。大多数幼虫的鱼鳔膨胀在4小时后孵化（HPH）在日本青鳉和斑马鱼在48 HPH的控制。在这两种鱼类中，暴露于PFBA（青鳉的最低观测效应浓度[LOEC]：40 mg/L;斑马鱼：80 mg/L）、TDCPP（青鳉的LOEC：1 mg/L;斑马鱼：0.5 mg/L）和T3（日本青鳉无抑制作用;斑马鱼的LOEC：7.5 μg/L）的幼虫的鱼鳔膨胀受到抑制。我们还检测了PFBA、TDCPP和T3对促甲状腺激素β亚基（tshβ）或甲状腺激素受体α（trα）和β（trβ）表达的影响。PFBA和TDCPP暴露后，基因表达没有观察到变化;然而，T3暴露上调两种鱼类的trα和trβ表达。当比较日本青鳉和斑马鱼的结果时，发现两种物种的鱼鳔膨胀都受到甲状腺激素干扰化学物质的抑制。我们的研究结果表明，在4小时/小时的日本青鳉和48小时/小时的斑马鱼中抑制鱼鳔膨胀是化学物质干扰甲状腺激素活性的潜在指标。
We compared the influence of thyroid hormone‐disrupting chemicals (heptafluorobutanoic acid, PFBA and tris[1,3‐dichloro‐2‐propyl] phosphate, TDCPP) and thyroid hormone (3,3′,5‐triiodo‐L‐thyronine, T3) on swim bladder inflation and thyroid hormone‐related gene expression in Japanese medaka and zebrafish. The swim bladder of most larvae had inflated at 4 h post hatching (hph) in Japanese medaka and at 48 hph in zebrafish in controls. In both fish species, the swim bladder inflation was inhibited in larvae exposed to PFBA (lowest observed effect concentration [LOEC] in medaka: 40 mg/L; in zebrafish: 80 mg/L), TDCPP (LOEC in medaka: 1 mg/L; in zebrafish: 0.5 mg/L), and T3 (no inhibition in Japanese medaka; LOEC in zebrafish: 7.5 μg/L). We also examined the influence of PFBA, TDCPP, and T3 on the expression of thyroid stimulating hormone subunit beta (tshβ) or thyroid hormone receptor alpha (trα) and beta (trβ). No changes were observed in the expression of genes after PFBA and TDCPP exposure; however, T3 exposure upregulated trα and trβ expression in both fish species. When the results were compared between Japanese medaka and zebrafish, swim bladder inflation in both species was found to be inhibited by exposure to thyroid hormone‐disrupting chemicals. Our results show that inhibition of the swim bladder inflation at 4 hph in Japanese medaka and 48 hph in zebrafish is a potential indicator of thyroid hormone‐disturbing activity of chemicals.