CD44 and its ligand hyaluronate mediate rolling under physiologic flow: a novel lymphocyte-endothelial cell primary adhesion pathway.
CD44 and its ligand hyaluronate mediate rolling under physiologic flow: a novel lymphocyte-endothelial cell primary adhesion pathway.
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DOI：
10.1084/jem.183.3.1119
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发表时间：
1996-03-01
期刊：
影响因子：
--
通讯作者：
Siegelman MH
中科院分区：
文献类型：
--
作者：
DeGrendele HC;Estess P;Picker LJ;Siegelman MH
白细胞从血液外渗到组织中是一个多步骤的过程：最初的短暂相互作用（“滚动”），通常认为是由粘附分子的选择素家族介导的，然后是牢固的粘附，通常由整联蛋白介导。使用平行板流动室设计近似生理流量在毛细血管后小静脉，我们的特点是滚动淋巴细胞和粘附的原代和培养的内皮细胞之间的相互作用，不是选择素介导的。使用阻断单克隆抗体的研究表明，这种新的相互作用是由CD 44介导的。使用可溶性透明质酸盐（HA）和用HA反应性物质处理贴壁细胞可以特异性地实现滚动相互作用的消除，这表明HA是支持这种滚动相互作用的配体。一些B和T细胞系以及正常淋巴细胞，或者组成性地表现出滚动，或者可以通过佛波酯或体内抗原活化诱导这样做。这些研究表明，CD 44及其主要配体透明质酸代表另一种受体/碳水化合物配体对介导淋巴细胞/内皮细胞粘附的新的活化依赖性途径。
The extravasation of leukocytes from the blood into tissues occurs as a multistep process: an initial transient interaction ("rolling"), generally thought to be mediated by the selectin family of adhesion molecules, followed by firm adhesion, usually mediated by integrins. Using a parallel plate flow chamber designed to approximate physiologic flow in postcapillary venules, we have characterized a rolling interaction between lymphoid cells and adherent primary and cultured endothelial cells that is not selectin mediated. Studies using blocking monoclonal antibodies indicate that this novel interaction is mediated by CD44. Abrogation of the rolling interaction could be specifically achieved using both soluble hyaluronate (HA) and treatment of the adherent cells with HA-reactive substances, indicating that HA is the ligand supporting this rolling interaction. Some B and T cell lines, as well as normal lymphocytes, either constitutively exhibit rolling or can be induced to do so by phorbol ester or in vivo antigen activation. These studies indicate that CD44 and its principal ligand hyaluronate represent another receptor/carbohydrate ligand pair mediating a novel activation-dependent pathway of lymphocyte/endothelial cell adhesion.