Multifactorial processes underlie parallel opsin loss in neotropical bats
Multifactorial processes underlie parallel opsin loss in neotropical bats
复制标题
DOI：
10.7554/elife.37412
复制
发表时间：
2018-12-18
期刊：
影响因子：
7.7
通讯作者：
Sears, Karen E.
中科院分区：
文献类型：
--
作者：
Sadier, Alexa;Davies, Kalina T. J.;Sears, Karen E.
先前适应性特征的丧失通常与选择中的放松有关，但导致这种重复丧失的分子步骤很少为人所知。基因缺失的分子研究往往只关注基因序列，而忽视蛋白质表达的其他方面可能会低估表型多样性。例如，几乎完全基于视蛋白基因进化的见解使哺乳动物的色觉成为表型丧失的教科书范例。我们解决这个差距，通过调查的保留和损失的视蛋白基因，转录本，和蛋白质在生态多样的蝙蝠。我们发现短波敏感视蛋白存在多重、独立的损失。推定功能性DNA序列、mRNA转录本和蛋白质之间的不匹配暗示了一些夜光蝙蝠中S-视蛋白持续丢失的转录和转录后过程。我们的研究结果提供了一个快照的进化过程中的表型性状的损失，并建议脊椎动物的视觉表型不能总是单独从基因型预测。
The loss of previously adaptive traits is typically linked to relaxation in selection, yet the molecular steps leading to such repeated losses are rarely known. Molecular studies of loss have tended to focus on gene sequences alone, but overlooking other aspects of protein expression might underestimate phenotypic diversity. Insights based almost solely on opsin gene evolution, for instance, have made mammalian color vision a textbook example of phenotypic loss. We address this gap by investigating retention and loss of opsin genes, transcripts, and proteins across ecologically diverse noctilionoid bats. We find multiple, independent losses of short-wave-sensitive opsins. Mismatches between putatively functional DNA sequences, mRNA transcripts, and proteins implicate transcriptional and post-transcriptional processes in the ongoing loss of S-opsins in some noctilionoid bats. Our results provide a snapshot of evolution in progress during phenotypic trait loss, and suggest vertebrate visual phenotypes cannot always be predicted from genotypes alone.