Heat-shock-induced color-pattern changes of the blue pansy butterfly Junonia orithya: Physiological and evolutionary implications
Heat-shock-induced color-pattern changes of the blue pansy butterfly Junonia orithya: Physiological and evolutionary implications
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DOI：
10.1016/j.jtherbio.2011.06.002
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发表时间：
2011-08-01
影响因子：
2.7
通讯作者：
Otaki, Joji M.
中科院分区：
文献类型：
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作者：
Mahdi, Shah H. A.;Yamasaki, Hideo;Otaki, Joji M.
温度对早期蛹的冲击会导致蝴蝶翅膀颜色图案的变化。这些可塑性变化归因于蛹中血淋巴中冷休克激素(CSH)的水平以及其他机制。在这里，我们用蓝色三色雀朱尼亚算法(鳞翅目：若蝇科)描述了热休克诱导的颜色模式变化。在38-42摄氏度的热休克处理中，旁焦元素(PFE)变薄并移位离开视点；这与冷休克诱导的变化方向相反。有些令人惊讶的是，在致命的44摄氏度热休克的反应中，全氟辛烷基醚被修改，就像冷休克的情况一样。这些修改不受切除蛹的头前胸部分的影响。在异种共生实验中，血淋巴介导的42℃处理可能诱导的皮氏变性转移不成功，而44℃处理诱导的因子转移成功。相比之下，蓝色背景面积的减少不仅通过42摄氏度和44摄氏度的处理，而且还通过在雄性体内注射植物来源的应激诱导剂thapsigargin来实现。这种处理的结果与其他亲缘关系密切的菊科植物的自然颜色模式相似。我们还观察到，42摄氏度的处理增加了女性的橙色，这种变化类似于蜕皮类固醇诱导的修饰。综上所述，热休克诱导的PFE的改变可以用CSH的水平来解释，而其他的改变可能是由一般的应激反应和蜕皮激素效应引起的。我们得出结论，机翼颜色模式对热冲击的表型可塑性是至少几种不同机制共同作用的结果。这些机制可能被利用在一些朱尼亚属物种的颜色模式进化中。(C)2011爱思唯尔有限公司。保留所有权利。
Temperature shock to early pupae causes wing color-pattern changes in butterflies. These plastic changes are ascribed to the hemolymph level of the cold-shock hormone (CSH) in pupae as well as to other mechanisms. Here, we characterized heat-shock-induced color-pattern changes using the blue pansy butterfly Junonia orithya (Lepidoptera: Nymphalidae). In response to the 38-42 degrees C heat-shock treatments, parafocal elements (PFEs) were thinned and dislocated away from eyespots; this was the reverse of the direction of the cold-shock-induced changes. Somewhat surprisingly, in response to the lethal 44 degrees C heat shock, PFEs were modified as in the case of a cold-shock. These modifications were not affected by the removal of the head-prothorax portion of pupae. While the hemolymph-mediated transfer of the possible PEE-modification property induced by the 42 degrees C treatment was unsuccessful in the parabiosis experiment, the transfer of the factor induced by the 44 degrees C treatment was successful. In contrast, reduction of the blue background area was obtained not only by the 42 and 44 degrees C treatments but also by the injection of thapsigargin, a plant-derived stress inducer, in males. The result of this treatment was similar to the natural color patterns of other closely related Junonia species. We also observed an increase in orange coloration by the 42 degrees C treatment in females, and this change was similar to ecdysteroid-induced modifications. Taken together, the heat-shock-induced PFE modifications in J. orithya can be explained by the levels of CSH, and other modifications are likely to be caused by general stress responses and ecdysteroid effects. We conclude that phenotypic plasticity of the wing color patterns to heat shock results from a combined effect of at least a few different mechanisms. These mechanisms might have been exploited in the color-pattern evolution of some Junonia species. (C) 2011 Elsevier Ltd. All rights reserved.