Droplet-based Biosensing for Lab-on-a-Chip, Open Microfluidics Platforms.
Droplet-based Biosensing for Lab-on-a-Chip, Open Microfluidics Platforms.
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DOI：
10.3390/bios6020014
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发表时间：
2016-04-14
期刊：
影响因子：
--
通讯作者：
Alam MA
中科院分区：
文献类型：
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作者：
Dak P;Ebrahimi A;Swaminathan V;Duarte-Guevara C;Bashir R;Alam MA
低成本便携式传感器可以通过向低收入和中等收入人口，特别是发展中国家的低收入和中等收入人口提供易于获得的诊断设备来改变卫生保健。样品制备、分析物处理和标记是传统实验室诊断系统的主要成本问题。基于液滴微流体的芯片实验室（LoC）平台有望将这些复杂而昂贵的实验室程序集成并自动化到单个芯片上；如果可以将无标签生物传感器集成到LoC平台上，则成本将进一步降低。在这里，我们回顾了一些与“开放”数字微流体系统兼容的无标签、基于液滴的生物传感器的最新进展。这些低成本的基于液滴的生物传感器克服了传统传感器的一些基本限制，能够及时诊断。我们确定了使这些传感器具有商业可行性必须解决的关键挑战，并总结了一些有前途的研究方向。
Low cost, portable sensors can transform health care by bringing easily available diagnostic devices to low and middle income population, particularly in developing countries. Sample preparation, analyte handling and labeling are primary cost concerns for traditional lab-based diagnostic systems. Lab-on-a-chip (LoC) platforms based on droplet-based microfluidics promise to integrate and automate these complex and expensive laboratory procedures onto a single chip; the cost will be further reduced if label-free biosensors could be integrated onto the LoC platforms. Here, we review some recent developments of label-free, droplet-based biosensors, compatible with “open” digital microfluidic systems. These low-cost droplet-based biosensors overcome some of the fundamental limitations of the classical sensors, enabling timely diagnosis. We identify the key challenges that must be addressed to make these sensors commercially viable and summarize a number of promising research directions.