Studies on the quantitative biology of hyperthermic killing of HeLa cells.
Studies on the quantitative biology of hyperthermic killing of HeLa cells.
复制标题
高温杀伤HeLa细胞的定量生物学研究。
作者：
R. J. Palzer;C. Heidelberger
本研究对HeLa细胞高温杀伤的定量生物学进行了研究。细胞存活率与热疗剂量的关系曲线未显示一级动力学。在41.0-43.0°温度范围内，HeLa细胞的杀伤率显示出显著的温度依赖关系。加热至42.0°的细胞分裂延迟约1天，之后一些细胞恢复正常生长，而另一些细胞在死亡前至少分裂一次。在随后的热处理过程中，因其在长时间高温下存活的能力而被选择的细胞以与先前未被加热的细胞大致相同的速率被杀死。在某些DNA和蛋白质合成抑制剂化合物存在下加热的细胞中，高温细胞杀伤减少。分次剂量实验表明，细胞从亚致死的高温损伤中恢复。此外，高温杀死至少是一个两步过程，并且细胞能够从潜在的致命损伤中恢复，特别是在放线菌酮和高水平胸苷的存在下。
Summary The quantitative biology of hyperthermic killing of HeLa cells was studied. Plots of cell survival versus doses of hyperthermia did not show first-order kinetics. The rate of HeLa cell killing shows a striking temperature-dependent relationship in the 41.0–43.0° temperature range. There is a delay of approximately 1 day in the division of cells heated to 42.0°, after which time some cells resume normal growth, whereas others divide at least once before death. Cells selected for their capacity to survive prolonged periods of hyperthermia are killed at approximately the same rate during subsequent heat treatments as cells that had not been heated previously. Hyperthermic cell killing is reduced in cells that are heated in the presence of certain compounds that are inhibitors of DNA and protein synthesis. Fractionated dose experiments indicate that cells recover from sublethal hyperthermic damage. Furthermore, hyperthermic killing is at least a two-step process, and cells are capable of recovery from potentially lethal damage, particularly in the presence of cycloheximide and high levels of thymidine.