Developmental and diel changes of adipokinetic hormone in CNS and haemolymph of the flightless wing-polymorphic bug, Pyrrhocoris apterus (L.).
Developmental and diel changes of adipokinetic hormone in CNS and haemolymph of the flightless wing-polymorphic bug, Pyrrhocoris apterus (L.).
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不会飞的翅多形虫 Pyrrocoris apterus (L.) 中枢神经系统和血淋巴中脂肪运动激素的发育和昼夜变化。
DOI：
10.1016/s0022-1910(02)00245-7
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发表时间：
2003
影响因子：
2.2
通讯作者：
Z. Syrová
中科院分区：
文献类型：
--
作者：
D. Kodrík;R. Socha;Z. Syrová
我们使用酶联免疫分析 (ELISA) 测定了成年大翅和短翅雌性 Pyrrocoris apterus 的中枢神经系统（大脑 + 贲门体 + 全体）和血淋巴中的 AKH 含量。测试显示，在成年后的前 14 天，AKH 含量在 1 到几乎 4 pmol/CNS 之间波动，并且按滞育短翅目 < 生殖短翅目 < 大型翅目的顺序显着增加。血淋巴中 AKH 的滴度大多仅在该时期的大翅雌性中检测到；在繁殖或滞育的短翅雌性中，其浓度大多保持在可检测限度以下——1 fmol/μl。 10日龄大翅雌性中枢神经系统和血淋巴中AKH含量在24 h内发生显着变化，分别在光照开始后10 h左右和光照期后半段达到最高值。 CNS中AKH含量的昼夜变化与AKH的脂肪运动反应的昼夜变化呈正相关。免疫组织化学显示几乎所有反应物质都集中在贲门体后部，只有少量的细胞群和轴突分布在整个大脑中。
We have used an enzyme-linked immunoassay (ELISA) for determination of the AKH content in CNS (brain + corpora cardiaca + corpora allata) and haemolymph of adult macropterous and brachypterous females of the bug Pyrrhocoris apterus. The tests revealed that the AKH content fluctuates between 1 and almost 4 pmol/CNS during the first 14 days of adult life and significantly increased in order diapausing brachypters<reproductive brachypters<macropters. The titre of AKH in haemolymph is detectable mostly only in macropterous females during that period; in reproductive or diapausing brachypterous females keeps mostly below the detectable limit—1 fmol/μl. There are significant changes of AKH content in CNS and haemolymph of 10-day-old macropterous females during a 24 h period, with the highest values at about 10 h after the onset of light and at second half of the light period, respectively. The diel changes of AKH content in CNS are positively correlated with diel changes of adipokinetic response to AKH. Immunohistochemistry revealed that almost all reactive material is localized in the posterior part of the corpora cardiaca, and just a small amount in a few cell groups and axons distributed over the whole brain.
脂肪运动激素刺激昆虫中枢神经系统中的神经元。
DOI：
10.1242/jeb.198.6.1307
发表时间：
1995
期刊：
The Journal of experimental biology
影响因子：
--
作者：
Milde,JJ;Ziegler,R;Wallstein,M
通讯作者：
Wallstein,M