Oxytocin and estrogen receptor alpha and beta knockout mice provide discriminably different odor cues in behavioral assays.
Oxytocin and estrogen receptor alpha and beta knockout mice provide discriminably different odor cues in behavioral assays.
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催产素和雌激素受体α和β基因敲除小鼠在行为测定中提供了明显不同的气味线索。
DOI：
10.1111/j.1601-183x.2004.00068.x
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发表时间：
2004
期刊：
影响因子：
--
通讯作者：
Ogawa,S
中科院分区：
文献类型：
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作者：
Kavaliers,M;Agmo,A;Choleris,E;Gustafsson,JA;Korach,KS;Muglia,LJ;Pfaff,DW;Ogawa,S
社会行为包括社会线索的识别和产生。选择性删除催产素（OT）基因或雌激素受体（ER）-α或-β基因的小鼠显示出社会识别受损。在这项研究中，我们证明这些基因敲除小鼠也在行为测定中提供了明显不同的社会刺激。在气味选择测试中，这是一种衡量社会兴趣和辨别力的方法，远系繁殖的雌性Swiss韦伯斯特小鼠区分了雄性基因敲除小鼠（KO：OTKO、αERKO、βERKO）的尿液气味与其野生型同窝小鼠（WT：OTWT、αERWT、βERWT）的气味。雌性动物对OTWT和βERWT雄性动物的尿液气味的初始选择明显优于OTKO和βERKO雄性动物，对α ERKO雄性动物的尿液气味的初始选择明显优于αERWT雄性动物。OTKO和βERKO雄性的气味也诱导了厌恶性、镇痛性反应，而WT的气味没有显著影响。αERWT和αERKO雄性动物的气味均诱导了厌恶性镇痛反应，WT的气味诱导了显著更强的镇痛作用。束缚应激的WT和KO雄性动物的气味也引起镇痛作用，同样，雌性动物对应激的OTKO和βERKO雄性动物的气味的反应显著高于其WT，对应激的αERKO气味的镇痛作用显著低于αERWT雄性动物。这些研究结果表明，KO小鼠的气味的基础上，从他们的WT和不同的科斯不同的气味的相对吸引力/厌恶。因此，在行为分析中，基因失活改变基因敲除小鼠的社会行为的一个因果途径可能是通过伴侣对它们气味的改变反应来介导的。
Social behavior involves both the recognition and production of social cues. Mice with selective deletion (knockout) of either the gene for oxytocin (OT) or genes for the estrogen receptor (ER) ‐α or ‐β display impaired social recognition. In this study we demonstrate that these gene knockout mice also provide discriminably different social stimuli in behavioral assays. In an odor choice test, which is a measure of social interest and discrimination, outbred female Swiss‐Webster mice discriminated the urine odors of male knockouts (KO: OTKO, αERKO, βERKO) from the odors of their wildtype littermates (WT: OTWT, αERWT, βERWT). Females showed marked initial choices of the urine odors of OTWT and βERWT males over those of OTKO and βERKO males, and αERKO males over αERWT males. The odors of OTKO and βERKO males also induced aversive, analgesic responses, with the odors of WTs having no significant effects. Odors of both the αERWT and αERKO males induced aversive, analgesic responses, with the odors of the WT inducing significantly greater analgesia. The odors of restraint stressed WT and KO males also elicited analgesia with, again, females displaying significantly greater responses to the odors of stressed OTKO and βERKO males than their WTs, and significantly lower analgesia to the odors of stressed αERKO than αERWT males. These findings show that the KO mice are discriminated from their WTs on the basis of odor and that the various KOs differ in the relative attractiveness/aversiveness of their odors. Therefore, in behavioral assays one causal route by which gene inactivation alters the social behavior of knockout mice may be mediated through the partners' modified responses to their odors.