Spinal nociceptive transmission by mechanical stimulation of bone marrow.
Spinal nociceptive transmission by mechanical stimulation of bone marrow.
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DOI：
10.1177/1744806916628773
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发表时间：
2016
期刊：
影响因子：
3.3
通讯作者：
Kawamata M
中科院分区：
文献类型：
--
作者：
Ishida T;Tanaka S;Sekiguchi T;Sugiyama D;Kawamata M
由于骨髓接受来自A-δ和C-纤维的神经支配，并且由于骨髓中髓内压力的增加可能在外科手术期间引起骨科患者的急性疼痛和在骨髓水肿患者中引起慢性疼痛，骨骼疼痛可能部分源自骨髓。骨内病变，如骨髓炎和骨癌，也被认为会产生皮肤过敏，这可能是骨骼引起的疼痛。然而，很少有人知道在骨髓疼痛知觉和涉及疼痛引起的骨疾病。因此，我们进行了体内电生理学研究和行为研究，以确定是否增加股骨骨内压引起急性疼痛，以及是否增加骨内压引起皮肤相应感受野的牵涉痛。骨内球囊膨胀引起腰骶部自发性疼痛相关行为和机械性痛觉过敏和异常性疼痛。细胞外分离脊髓背角神经元的单个神经元活动，然后记录对非伤害性和伤害性皮肤刺激和骨内球囊膨胀的诱发反应。获得了94个脊髓背角神经元，其在下背部和大腿处具有躯体感受野。62%的宽动态范围神经元（24/39）和86%的高阈值神经元（12/14）对骨内球囊膨胀有反应，而低阈值神经元（0/41）对骨内球囊膨胀无反应。脊髓给药吗啡（1 µg）可消除清醒大鼠球囊扩张诱导的自发性疼痛相关行为和机械性痛觉过敏，还可抑制宽动态范围神经元对伤害性皮肤刺激和骨内球囊扩张的诱发活动。结果表明，机械刺激骨髓产生伤害性感受，同时在相应的皮肤区域产生牵涉痛。这些机制可能会导致骨骼疾病引起的疼痛。
Since bone marrow receives innervation from A-delta and C-fibers and since an increase in intramedullary pressure in bone marrow may induce acute pain in orthopedic patients during surgery and chronic pain in patients with bone marrow edema, skeletal pain may partly originate from bone marrow. Intraosseous lesions, such as osteomyelitis and bone cancer, are also known to produce cutaneous hypersensitivity, which might be referred pain from bone. However, little is known about pain perception in bone marrow and referred pain induced by bone disease. Thus, we carried out an in vivo electrophysiological study and behavioral study to determine whether increased intraosseous pressure of the femur induces acute pain and whether increased intraosseous pressure induces referred pain in the corresponding receptive fields of the skin. Intraosseous balloon inflation caused spontaneous pain-related behavior and mechanical hyperalgesia and allodynia in the lumbosacral region. Single neuronal activities of spinal dorsal horn neurons were extracellularly isolated, and then evoked responses to non-noxious and noxious cutaneous stimuli and intraosseous balloon inflation were recorded. Ninety-four spinal dorsal horn neurons, which had somatic receptive fields at the lower back and thigh, were obtained. Sixty-two percent of the wide-dynamic-range neurons (24/39) and 86% of the high-threshold neurons (12/14) responded to intraosseous balloon inflation, while none of the low-threshold neurons (0/41) responded to intraosseous balloon inflation. Spinally administered morphine (1 µg) abolished balloon inflation-induced spontaneous pain-related behavior and mechanical hyperalgesia in awake rats and also suppressed evoked activities of wide-dynamic-range neurons to noxious cutaneous stimulation and intraosseous balloon inflation. The results suggest that mechanical stimulation to bone marrow produces nociception, concomitantly producing its referred pain in the corresponding skin fields. These mechanisms might contribute to pain caused by skeletal diseases.