Titanium implants induce expression of matrix metalloproteinases in bone during osseointegration.
Titanium implants induce expression of matrix metalloproteinases in bone during osseointegration.
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DOI：
10.1682/jrrd.2003.07.0107
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发表时间：
2004-11
影响因子：
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通讯作者：
V. Shubayev;R. Brånemark;J. Steinauer;R. Myers
中科院分区：
文献类型：
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作者：
V. Shubayev;R. Brånemark;J. Steinauer;R. Myers
植入的纯钛固定装置能够与骨完全结合，部分原因是在钛-骨界面形成了牢固的细胞外基质（ECM）结合。在这项研究中，我们使用啮齿动物股骨髓内骨整合模型来分析ECM控制基质金属蛋白酶（MMPs），金属蛋白酶组织抑制剂-3（TIMP-3）和肿瘤坏死因子α（TNF-α）在骨整合过程中的免疫反应性变化。我们观察到骨细胞、破骨细胞、哈弗氏管和钛种植体同侧骨界面基质中MMP-2、MMP-9、MMP-7、TIMP-3和TNF-α的显著增加。在实验骨中还观察到肥大软骨细胞和骺生长板血管成分中TIMP-3、MMP-9和MMP-7的增加。在对侧或假手术骨中未观察到这些发现，其中钛固定装置被拧入股骨并立即取出。我们的数据链接钛诱导的骨重建的变化，在表达和分布的基质金属蛋白酶。
Implanted pure titanium fixtures are able to completely integrate with bone, in part because of the formation of a strong extracellular matrix (ECM) bond at the titanium-bone interface. In this study, we used a rodent femur model of intramedullary osseointegration to analyze the changes in immunoreactivity of ECM-controlling matrix metalloproteinases (MMPs), tissue inhibitor of metalloproteinase-3 (TIMP-3), and tumor necrosis factor alpha (TNF-alpha) during osseointegration. We observed dramatic increases in MMP-2, MMP-9, MMP-7, TIMP-3, and TNF-alpha in osteocytes, osteoclasts, haversian canals, and the interface matrix in bone ipsilateral to the titanium implant. An increase in TIMP-3, MMP-9, and MMP-7 in hypertrophied chondrocytes and the vascular component of the epiphysial growth plate was also observed in experimental bone. These findings were not seen in contralateral or sham-operated bone, where the titanium fixtures were threaded into the femur and immediately removed. Our data link titanium-induced bone remodeling to changes in expression and distribution of MMPs.