Sharing Structure and Function in Biological Design with SBOL 2.0
Sharing Structure and Function in Biological Design with SBOL 2.0
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DOI：
10.1021/acssynbio.5b00215
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发表时间：
2016-06-01
影响因子：
4.7
通讯作者：
Myers, Chris J.
中科院分区：
文献类型：
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作者：
Roehner, Nicholas;Beal, Jacob;Myers, Chris J.
合成生物学开放语言（SBOL）是一种标准，可以在不同的机构和工具之间实现生物系统的协作工程。SBOL是通过仔细考虑最近的合成生物学趋势，真实的用例以及该领域领先研究人员和商业生物技术企业成员之间的共识而开发的。我们演示并讨论了一组支持SBOL的软件工具如何形成一个集成的跨组织工作流程，以概括迄今为止最大的已发表基因电路之一的设计，即4输入AND传感器。这种设计包括系统的结构组件，如DNA、RNA、小分子和蛋白质，以及决定系统行为/功能的这些组件之间的相互作用。所展示的工作流程和由此产生的电路设计说明了SBOL 2.0在自动交换遗传部件、设备及其操作的生物系统的结构和功能规范方面的实用性。
The Synthetic Biology Open Language (SBOL) is a standard that enables collaborative engineering of biological systems across different institutions and tools. SBOL is developed through careful consideration of recent synthetic biology trends, real use cases, and consensus among leading researchers in the field and members of commercial biotechnology enterprises. We demonstrate and discuss how a set of SBOL-enabled software tools can form an integrated, cross-organizational workflow to recapitulate the design of one of the largest published genetic circuits to date, a 4-input AND sensor. This design encompasses the structural components of the system, such as its DNA, RNA, small molecules, and proteins, as well as the interactions between these components that determine the system's behavior/function. The demonstrated workflow and resulting circuit design illustrate the utility of SBOL 2.0 in automating the exchange of structural and functional specifications for genetic parts, devices, and the biological systems in which they operate.