Nuclear traffic in fungal hyphae: in vivo study of nuclear migration and positioning in Aspergillus nidulans
Nuclear traffic in fungal hyphae: in vivo study of nuclear migration and positioning in Aspergillus nidulans
复制标题
DOI：
10.1046/j.1365-2958.1997.5131873.x
复制
发表时间：
1997-08-01
影响因子：
3.6
通讯作者：
Fischer, R
中科院分区：
文献类型：
--
作者：
Suelmann, R;Sievers, N;Fischer, R
核迁移和核定位是所有真核细胞的基本过程。在丝状真菌的菌丝生长过程中很容易监测它们。我们将绿色荧光蛋白 (GFP) 表达为与构巢曲霉细胞核中转录激活因子 StuA 的假定核定位域的融合蛋白，并在活细胞中可视化这些细胞器。在间期细胞核中观察到核染色，但在有丝分裂期间未观察到。在菌丝延伸过程中，用延时视频显微镜分析核分裂、核迁移、隔膜形成和分支。菌丝以 0.1-1.2 μ m min(-1) 的速度伸长，细胞核以相似的速度向菌丝尖端移动，直到到达指定位置。观察到给定菌丝区室中核流动性的个体调节。一些有代表性的电影可以在互联网上找到（http://www.blacksci.co.uk/products/journals/molextra.htm）。核定位也在分子狂欢中进行了研究。通过免疫荧光和 GFP 标记，调节核迁移的 ApsA 蛋白位于幼体和菌丝的细胞质膜上。提出了核迁移、核定位和 ApsA 作用的模型。
Nuclear migration and nuclear positioning are fundamental processes in all eukaryotic cells. They are easily monitored during hyphal growth of filamentous fungi. We expressed the green fluorescent protein (GFP) as a fusion protein with the putative nuclear localization domain of the transcriptional activator stuA in nuclei of Aspergillus nidulans and visualized these organelles in living cells. Nuclear staining was observed in interphase nuclei but not during mitosis. Nuclear division, nuclear migration, septum formation and branching were analysed with time-lapse video microscopy during hyphal extension. Hyphae elongated at 0.1-1.2 mu m min(-1) and nuclei moved with similar speeds towards the hyphal tip until they had reached a defined position. An individual regulation of nuclear mobility in a given hyphal compartment was observed. Some representative movies are available on the Internet (http://www.blacksci.co.uk/products/journals/molextra.htm). Nuclear positioning was also studied at the molecular revel. The ApsA protein, which regulates nuclear migration, was localized at the cytoplasmic membrane in germlings and hyphae by immunofluorescence and GFP tagging. A model of nuclear migration, nuclear positioning and the role of ApsA is presented.