The acidic, glutamine-rich Mpn474 protein of Mycoplasma pneumoniae is surface exposed and covers the complete cell
The acidic, glutamine-rich Mpn474 protein of Mycoplasma pneumoniae is surface exposed and covers the complete cell
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DOI：
10.1099/mic.0.2007/013342-0
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发表时间：
2008-04-01
期刊：
影响因子：
2.8
通讯作者：
Herrmann, Richard
中科院分区：
文献类型：
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作者：
Hegermann, Jan;Halbedel, Sven;Herrmann, Richard
人致病性肺炎支原体mpn474基因编码的Mpn474蛋白含有1033个氨基酸，等电点为4.79，是由于谷氨酸残基过多(11%)所致。虽然蛋白质缺乏可识别的输出信号，但我们通过免疫电子显微镜显示Mpn474表面暴露，完全覆盖了细胞。通过将Triton X-100与细菌细胞进行交联和仔细处理，我们发现该蛋白与细胞表面结合较弱，而在相同的实验条件下，真正的跨膜蛋白lMpn141(粘附素P1)牢固地附着在细胞表面。Mpn474基因的一个无明显表型的转座子突变作为免疫检测的阴性对照。
The protein Mpn474 encoded by the mpn474 gene of the human-pathogenic Mycoplasma pneumoniae contains 1033 amino acids and has an isoelectric point of 4.79, which is caused by the large excess of glutamic acid residues (11%). Although the protein lacks recognizable export signals we showed by immuno-electron microscopy that Mpn474 is surface exposed, covering the cell completely. By combining cross-linking and careful treatment of the bacterial cells with Triton X-100, we found that this protein is weakly bound to the cell surface, while the true transmembrane protein lMpn141 (adhesin P1) is firmly attached under the same experimental conditions. A transposon mutant in the mpn474 gene, which has no obvious phenotype, served as negative control for the immunodetection.