Finding a universal low viscosity polymer for DNA separation (II)
Finding a universal low viscosity polymer for DNA separation (II)
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DOI：
10.1002/elps.1150191813
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发表时间：
1998-12-01
期刊：
影响因子：
2.9
通讯作者：
Heller, C
中科院分区：
文献类型：
--
作者：
Heller, C
在研究不同聚合物在毛细管电泳中的应用时，我们发现与具有相当分子量和分辨率的其他聚合物相比，聚 N,N-二甲基丙烯酰胺 (pDMA) 的粘度非常低。这使得它成为多毛细管电泳中 DNA 分离的潜在有用基质，其中优选短循环时间或低压基质更换。我们通过对浓度、链长和场强依赖性的系统研究来表征该矩阵。结果表明，pDMA 对于寡核苷酸和双链 DNA 片段的分离表现良好。与 DNA 测序的应用一起，pDMA 成为一种用于分离生物大分子的通用聚合物。
When investigating the use of different polymers for capillary electrophoresis we found that poly-N,N-dimethylacrylamide (pDMA) has a very low viscosity compared to other polymers of comparable molecular mass and resolving power. This makes it a potentially useful matrix for DNA separation in multi-capillary electrophoresis, where short cycle times or low pressure for matrix replacement are preferred. We have characterized this matrix by systematic studies on concentration, chain length and field strength dependence. It is shown that pDMA performs well for the separation of oligonucleotides and double-stranded DNA fragments. Together with the application of DNA sequencing, pDMA is a universal polymer for the separation of biological macromolecules.