Secretome Profiling Reveals Virulence-Associated Proteins of Fusarium proliferatum during Interaction with Banana Fruit
Secretome Profiling Reveals Virulence-Associated Proteins of Fusarium proliferatum during Interaction with Banana Fruit
复制标题
分泌组分析揭示了增殖镰刀菌与香蕉果实相互作用过程中的毒力相关蛋白
DOI：
10.3390/biom9060246
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发表时间：
2019-06-01
期刊：
影响因子：
5.5
通讯作者：
Jiang, Yueming
中科院分区：
文献类型：
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作者：
Li, Taotao;Wu, Yu;Jiang, Yueming
分泌蛋白通过操纵宿主进行有效定殖，对许多真菌的致病性至关重要。增殖镰刀菌是一种植物病原真菌，感染许多农作物、蔬菜和水果，包括香蕉果实。为了获取参与病原体与宿主相互作用的蛋白质，我们使用无标记定量蛋白质组学技术来比较分析在 Czapek 肉汤培养基中添加和不添加香蕉皮培养的 F. proliferatum 的分泌蛋白组。通过分析 F. proliferatum 的分泌蛋白组，我们鉴定了 105 种蛋白质，其中 40 种是专门分泌的，65 种是因香蕉皮的反应而丰度增加的。这些蛋白质参与促进香蕉果实的侵袭，主要分为毒力因子、细胞壁降解、代谢过程、应激反应、调节以及其他未知的生物过程。相应基因的表达证实了香蕉皮中这些分泌蛋白的存在。此外，表达模式表明这些基因在不同感染阶段的作用不同。这项研究扩展了目前的 F. proliferatum 分泌蛋白数据库，这些蛋白可能与这种真菌的感染策略有关。此外，这项研究值得进一步关注一些可能引发香蕉果实上 F. proliferatum 感染的分泌蛋白。
Secreted proteins are vital for the pathogenicity of many fungi through manipulating their hosts for efficient colonization. Fusarium proliferatum is a phytopathogenic fungus infecting many crops, vegetables, and fruit, including banana fruit. To access the proteins involved in pathogen–host interaction, we used label-free quantitative proteomics technology to comparatively analyze the secretomes of F. proliferatum cultured with and without banana peel in Czapek’s broth medium. By analyzing the secretomes of F. proliferatum, we have identified 105 proteins with 40 exclusively secreted and 65 increased in abundance in response to a banana peel. These proteins were involved in the promotion of invasion of banana fruit, and they were mainly categorized into virulence factors, cell wall degradation, metabolic process, response to stress, regulation, and another unknown biological process. The expressions of corresponding genes confirmed the existence of these secreted proteins in the banana peel. Furthermore, expression pattern suggested variable roles for these genes at different infection stages. This study expanded the current database of F. proliferatum secreted proteins which might be involved in the infection strategy of this fungus. Additionally, this study warranted the further attention of some secreted proteins that might initiate infection of F. proliferatum on banana fruit.