The FXYD gene family of small ion transport regulators or channels: cDNA sequence, protein signature sequence, and expression
The FXYD gene family of small ion transport regulators or channels: cDNA sequence, protein signature sequence, and expression
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DOI：
10.1006/geno.2000.6274
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发表时间：
2000-08-15
期刊：
影响因子：
4.4
通讯作者：
Rael, E
中科院分区：
文献类型：
--
作者：
Sweadner, KJ;Rael, E
通过对1000多个相关EST（expressed sequence tags）的分析，确定了一个以磷膜蛋白和Na，K-ATP酶γ亚基为代表的小膜蛋白基因家族。除了已知家族成员（包括MAT-8，CHIF和RIC）的新的和更完整的cDNA序列外，研究结果还包括两个新的家族成员和新的剪接变体。大量的EST重复使得有可能以比单个克隆测序更高的置信度和准确性获得策展的DNA序列。该家族具有以单个跨膜跨度为中心的35个不变和保守氨基酸的核心基序。比较每种预测蛋白产物的特征，并确定组织分布。该基因家族被命名为FXYD（发音为fix-id），以识别其特征基序中的不变氨基酸，丰富的蛋白质参与控制离子转运。(C)北京大学出版社.
A gene family of small membrane proteins, represented by phospholemman and the gamma subunit of Na,K-ATPase, was defined and characterized by the analysis of more than 1000 related ESTs (expressed sequence tags). In addition to new and more complete cDNA sequence for known family members (including MAT-8, CHIF, and RIC), the findings included two new family members and new splicing variants. A large number of EST replicates made it possible to derive curated DNA sequence with higher confidence and accuracy than from the sequencing of individual clones. The family has a core motif of 35 invariant and conserved amino acids centered on a single transmembrane span. Features of each predicted protein product were compared, and tissue distributions were determined. The gene family was named FXYD (pronounced fix-id) in recognition of invariant amino acids in its signature motif, The abundant proteins are involved in the control of ion transport. (C) 2000 Academic Press.