Relationship between flavonoid structure and reactive oxygen species generation upon ultraviolet and X-ray irradiation
Relationship between flavonoid structure and reactive oxygen species generation upon ultraviolet and X-ray irradiation
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DOI：
10.1016/j.jphotochem.2019.112044
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发表时间：
2019-11-01
影响因子：
4.3
通讯作者：
Takahashi, Junko
中科院分区：
文献类型：
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作者：
Jiang, Lei;Yanase, Emiko;Takahashi, Junko
在这项研究中，我们的目的是揭示结构相关的活性氧（ROS）产生的类黄酮紫外线（UV）或X射线照射。利用ROS荧光探针研究了所选黄酮类化合物的ROS产生。讨论了黄酮类化合物的结构与超氧阴离子自由基（O-2（中心点-））、羟基自由基（（OH）-O-中心点）和单线态氧（O-1（2））生成的关系。结果表明，2，3-双键是天然黄酮类化合物生成O-2（中心点-）的关键结构。我们还通过氧自由基吸收能力（ORAC）测试讨论了ROS清除能力对ROS产生的影响。槲皮素衍生物清除活性氧能力不同，可产生（OH）-O-中心点。我们的研究结果表明，黄酮类化合物及其衍生物，这是用来作为抗氧化剂，可以作为ROS发生器后，紫外线（UV）或X射线照射。
In this research, we aimed to reveal structures related to reactive oxygen species (ROS) generation of flavonoids upon ultraviolet (UV) or X-ray irradiation. ROS production of selected flavonoids was studied by using ROS fluorescence probes. We discuss the relationship between the structures of the flavonoids and the generation of three ROSs, superoxide anion radical (O-2(center dot-)), hydroxyl radical ((OH)-O-center dot), and singlet oxygen (O-1(2)). Results showed that 2,3-double bond was an key structure for natural flavonoids to generate O-2(center dot-). We also discuss the effects of the ROS scavenging capacities on ROS generation through oxygen radical absorbance capacity (ORAC) tests. Derivatives of quercetin could produce (OH)-O-center dot due to differences of ROS scavenging ability. Our results indicated that flavonoids and their derivatives, which were used as antioxidant reagents, can serve as ROS generators upon ultraviolet (UV) or X-ray irradiation.