CAMP-INDEPENDENT CONTROL OF SPORULATION, GLYCOGEN-METABOLISM, AND HEAT-SHOCK RESISTANCE IN S-CEREVISIAE
CAMP-INDEPENDENT CONTROL OF SPORULATION, GLYCOGEN-METABOLISM, AND HEAT-SHOCK RESISTANCE IN S-CEREVISIAE
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DOI：
10.1016/0092-8674(88)90572-7
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发表时间：
1988-05-20
期刊：
影响因子：
64.5
通讯作者：
WIGLER, M
中科院分区：
文献类型：
--
作者：
CAMERON, S;LEVIN, L;WIGLER, M
编码cAMP依赖性蛋白激酶（cAPK）的调节亚基（BCY 1）和催化亚基（TPK 1、TPK 2和TPK 3）的基因在S.酿酒酵母、bcy 1-酵母菌株对营养条件没有适当的响应。与野生型不同，bcy 1-菌株不积累糖原，形成孢子，或在营养有限时对热休克具有抗性。我们已经分离出了抑制所有bcy 1缺陷的突变TPK基因。突变TPK基因似乎编码cAPK的功能减弱的催化亚基。bcy 1-酵母菌株含有突变TPK基因适当地响应营养条件，即使在缺乏CDC 25，两个RAS基因，或CYR 1。这些基因共同编码cAMP生成机制的已知组分。这些结果表明，不依赖环腺苷酸的机制在调节糖原积累、孢子形成和获得耐热性方面是存在的。啤酒。
Genes encoding the regulatory (BCY1) and catalytic (TPK1, TPK2, and TPK3) subunits of the cAMP-dependent protein kinase (cAPK) are found in S. cerevisiae, bcy1- yeast strains do not respond properly to nutrient conditions. Unlike wild type, bcy1- strains do not accumulate glycogen, form spores, or become resistant to heat shock when nutrient limited. We have isolated mutant TPK genes that suppress all of the bcy1- defects. The mutant TPK genes appear to encode functionally attenuated catalytic subunits of the cAPK. bcy1- yeast strains containing the mutant TPK genes respond appropriately to nutrient conditions, even in the absence of CDC25, both RAS genes, or CYR1. Together, these genes encode the known components of the cAMP-generating machinery. The results indicate that cAMP-independent mechanisms must exist for regulating glycogen accumulation, sporulation, and the acquisition of thermotolerance in S. cerevisiae.