Sensing of oligopeptides using localized surface plasmon resonances combined with Surface-Assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry.
Sensing of oligopeptides using localized surface plasmon resonances combined with Surface-Assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry.
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DOI：
10.1039/c1nr10519a
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发表时间：
2011-09
期刊：
影响因子：
6.7
通讯作者：
Y. Nakamura;Y. Tsuru;M. Fujii;Y. Taga;Ayaka Kiya;N. Nakashima;Y. Niidome
中科院分区：
文献类型：
--
作者：
Y. Nakamura;Y. Tsuru;M. Fujii;Y. Taga;Ayaka Kiya;N. Nakashima;Y. Niidome
将金纳米棒固定在ITO平板上，用于寡肽(血管紧张素I)的光谱传感和表面辅助激光解吸电离飞行时间质谱仪(SALDI-MS)。金纳米棒的纵向表面等离子激元带对浇铸在ITO平板上的10(-10)M血管紧张素溶液作出反应。Saldi-MS测量对ITO平板上的血管紧张素具有超高的灵敏度。在m/z=1297时，血管紧张素的表面密度很小(5×10~(-19)摩尔·厘米~(-2))，具有良好的信噪比(S/N=11)。用金纳米棒修饰的ITO平板被发现能够有效地收集金纳米棒附近的血管紧张素分子，并且金纳米棒的光吸收诱导的Saldi过程有效地促进了血管紧张素的解吸和电离。
Gold nanorods were fixed on an ITO plate and used for the spectroscopic sensing and Surface-Assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry (SALDI-MS) of oligopeptides (angiotensin I). The longitudinal surface plasmon bands of the gold nanorods responded to the 10(-10) M angiotensin solution that was cast on the ITO plate. The SALDI-MS measurements had an ultra-high sensitivity to the angiotensin on the ITO plate. A very small surface density (5 × 10(-19) mol cm(-2)) of angiotensin could be detected at m/z = 1297 with a good signal/noise ratio (S/N = 11). The ITO plate, which was modified with gold nanorods, was found to be effective in collecting angiotensin molecules adjacent to the gold nanorods, and the SALDI processes that were induced by the photoabsorption of the gold nanorods efficiently contributed to the desorption and ionization of the angiotensin.