Cytokeratin-positive subserosal myofibroblasts in gastroduodenal ulcer; another type of myofibroblasts
Cytokeratin-positive subserosal myofibroblasts in gastroduodenal ulcer; another type of myofibroblasts
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胃十二指肠溃疡中细胞角蛋白阳性浆膜下肌成纤维细胞；
DOI：
10.14670/hh-21.697
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发表时间：
2006-07-01
影响因子：
2
通讯作者：
Enzan, H
中科院分区：
文献类型：
--
作者：
Guo, L;Kuroda, N;Enzan, H
探讨人胃十二指肠溃疡穿孔中α-平滑肌肌动蛋白（ASMA）阳性基质细胞的分布和来源。对24例手术切除的胃十二指肠溃疡穿孔病灶进行免疫组化检查ASMA、HCD、CD34、CD31、CAM5.2和HMW-CK，并进行ASMA和CAM5.2双染色。此外，为了确定胶原蛋白的细胞来源，进行了胶原蛋白1 mRNA的原位杂交。在正常胃十二指肠壁中，在粘膜肌层、粘膜下层、固有肌层和浆膜下层中发现了 CD34 阳性基质细胞的网状网络。在上皮下区域，观察到许多肌成纤维细胞，而没有看到CD34阳性基质细胞。在溃疡性病变附近的区域，没有观察到 CD34 阳性基质细胞，但那里存在大量的肌成纤维细胞。在溃疡深层，穿孔周围浆膜下区域观察到大量ASMA和CAM5.2强阳性的梭形或星状基质细胞。在同一位点，通过双染色鉴定出许多共表达ASMA和CAM5.2的细胞。相反，在溃疡的表层中，观察到仅表达ASMA的基质细胞。人胃十二指肠溃疡中细胞角蛋白阳性浆膜下肌成纤维细胞是一种新型肌成纤维细胞。
To investigate the distribution and origin of alpha-smooth muscle actin (ASMA)-positive stromal cells in the perforation of human gastroduodenal ulcers. Perforative lesions of 24 surgically resected gastroduodenal ulcers were examined immunohistochemically for ASMA, HCD, CD34, CD31, CAM5.2 and HMW-CK, and double staining of ASMA and CAM5.2 was also performed. In addition, to determine the cell source of collagen, in situ hybridization of collagen 1 mRNA was performed. In the normal gastroduodenal wall, the reticular network of CD34-positive stromal cells was identified in the muscularis mucosa, submucosa, muscular propria, and subserosa. In the subepithelial area, many myofibroblasts were observed, whereas no CD34-positive stromal cells were seen. In areas neighboring ulcerative lesions, no CD34-positive stromal cells were observed, but a significant number of myofibroblasts were present there. In the deep layer of ulceration, numerous fusiform or stellate stromal cells strongly positive for ASMA and CAM5.2 were observed in the subserosal area around the perforation. In the same site, many cells co-expressing ASMA and CAM5.2 were identified by double staining. In contrast, in the surface layer of ulceration, stromal cells expressing only ASMA were observed. The cytokeratin-positive subserosal myofibroblastic cell in human gastroduodenal ulcer is a novel type of myofibroblast.