Identification of Phosphorylated Proteins on a Global Scale.
Identification of Phosphorylated Proteins on a Global Scale.
复制标题
DOI：
10.1002/cpch.48
复制
发表时间：
2018-09
影响因子：
--
通讯作者：
Iliuk A
中科院分区：
文献类型：
--
作者：
Iliuk A
液相色谱（LC）与串联质谱（MS/MS）相结合，使研究人员能够以前所未有的深度分析复杂的生物样品。它有助于在单个大规模蛋白质组学实验中鉴定和定量数千种蛋白质中的修饰。磷酸化分析作为最常见和最重要的翻译后修饰之一，尤其受益于这一领域的进展。在这里，我提供了几个公认的常见样品制备方法的有效磷酸化蛋白质组学实验的详细协议。
Liquid chromatography (LC) coupled with tandem mass spectrometry (MS/MS) has enabled researchers to analyze complex biological samples with unprecedented depth. It facilitates the identification and quantification of modifications within thousands of proteins in a single large-scale proteomic experiment. Phosphorylation analysis, as one of the most common and important post-translational modifications, has particularly benefited from such progress in the field. Here, I provide detailed protocols for a few well-regarded common sample preparation methods for an effective phosphoproteomic experiment.