Fontibacter ferrireducens sp. nov., an Fe(III)-reducing bacterium isolated from a microbial fuel cell.
Fontibacter ferrireducens sp. nov., an Fe(III)-reducing bacterium isolated from a microbial fuel cell.
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DOI：
10.1099/ijs.0.040998-0
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发表时间：
2013-03
影响因子：
2.8
通讯作者：
Jun Zhang;Guiqin Yang;Shungui Zhou;Yue-qiang Wang;Yong Yuan;Zhuang Li
中科院分区：
文献类型：
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作者：
Jun Zhang;Guiqin Yang;Shungui Zhou;Yue-qiang Wang;Yong Yuan;Zhuang Li
从微生物燃料电池中分离出一种新型的异化铁（III）还原细菌，命名为菌株SgZ-2(T)，它可以将葡萄糖氧化与铁还原结合起来以节约能源。革兰氏染色阴性，过氧化氢酶阳性，氧化酶阴性，兼性厌氧。菌株能在15-37℃、0- 5% (w/v) NaCl的条件下在胰蛋白酶琼脂上生长。主要细胞脂肪酸为iso-C15: 0、iso-C17: 0 3-OH和iso-C17: 1ω9c；呼吸醌类以MK-7为主；主要极性脂质为磷脂酰乙醇胺、一种未识别的氨基脂质和另外三种未识别的脂质；DNA G+C含量为55.3% mol%。基于16S rRNA序列的系统发育分析表明，该菌株与Fontibacter flavus CC-GZM-130(T)亲缘关系最近（序列相似性为99.9%），属于环杆菌科（Cyclobacteriaceae）。菌株SgZ-2(T)与F. flavus CCM 7650(T)的dna亲缘性（< 41%）表明，这两株菌株属于两个不同的物种。基于系统发育分析和表型特征，菌株SgZ-2(T)代表了Fontibacter属的一个新种，建议将其命名为Fontibacter ferrireducens sp. 11。型应变为SgZ-2(T) (= CCTCC M 2011498（T) = KACC 16525(T)）。
A novel, dissimilatory Fe(III)-reducing bacterium, designated strain SgZ-2(T), which could couple glucose oxidation to iron reduction for energy conservation, was isolated from a microbial fuel cell. The isolate was Gram-staining-negative, catalase-positive, oxidase-negative and facultatively anaerobic. The strain was able to grow on tryptic soy agar at 15-37 °C and in the presence of 0-5 % (w/v) NaCl. The predominant cellular fatty acids (>5 %) were iso-C15 : 0, iso-C17 : 0 3-OH and iso-C17 : 1ω9c; the major respiratory quinone was MK-7; the major polar lipids were phosphatidylethanolamine, an unidentified aminolipid and three other unidentified lipids; and the DNA G+C content was 55.3 mol%. Phylogenetic analyses based on 16S rRNA sequences showed that the novel strain was most closely related to Fontibacter flavus CC-GZM-130(T) (99.9 % sequence similarity), and was a member of the family Cyclobacteriaceae. The levels of DNA-DNA relatedness observed between strain SgZ-2(T) and F. flavus CCM 7650(T) (<41 %) indicated that the two strains represented two distinct species. Based on phylogenetic analyses and phenotypic characteristics, strain SgZ-2(T) represents a novel species of the genus Fontibacter, for which the name Fontibacter ferrireducens sp. nov. is proposed. The type strain is SgZ-2(T) ( = CCTCC M 2011498(T) = KACC 16525(T)).