Nerve-mast cell (RBL) interaction: RBL membrane ruffling occurs at the contact site with an activated neurite.
Nerve-mast cell (RBL) interaction: RBL membrane ruffling occurs at the contact site with an activated neurite.
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DOI：
10.1152/ajpcell.00050.2002
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发表时间：
2002-12
期刊：
影响因子：
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通讯作者：
N. Mori;R. Suzuki;T. Furuno;D. McKay;M. Wada;R. Teshima;J. Bienenstock;M. Nakanishi
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文献类型：
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作者：
N. Mori;R. Suzuki;T. Furuno;D. McKay;M. Wada;R. Teshima;J. Bienenstock;M. Nakanishi
肥大细胞-神经突相互作用作为神经免疫相互作用的模型。我们已经表明，神经突肥大细胞通信可以通过P物质与粘膜肥大细胞样细胞，大鼠嗜碱性白血病（RBL）细胞上的神经激肽（NK）-1受体相互作用发生。建立神经突（鼠上级颈神经节）和RBL细胞[表达颗粒相关抗原CD 63-绿色荧光蛋白（GFP）缀合物]共培养物，并用缓激肽（BK; 10 nM）或蝎毒（SV; 10 pg/ml）刺激，这两种物质都只激活神经突。通过Ca 2+的共聚焦成像（细胞预装载有fluo 3）评估细胞活化，并进行RBL CD 63-GFP+颗粒运动的分析。BK或SV激活神经突后，RBL Ca 2+动员，这是由NK-1受体拮抗剂（NK-1 RA）抑制。此外，膜皱褶上观察到RBL伪足延长与激活的神经突接触，但不对非接触伪足。NK-1 RA可抑制RBL膜皱褶，但NK-2 RA不抑制，并伴有颗粒运动的显著增加（0.13 +/- 0.04 vs. 0.05 +/- 0.01 microm/s），这在神经突接触点最明显：许多颗粒移向质膜。这是第一次记录神经和肥大细胞之间如此精确（仅限于膜的接触部位）的信息传递，这可以允许这两种细胞类型之间进行非常微妙的体内通讯。
Mast cell-neurite interaction serves as a model for neuroimmune interaction. We have shown that neurite-mast cell communication can occur via substance P interacting with neurokinin (NK)-1 receptors on the mucosal mast cell-like cell, the rat basophilic leukemia (RBL) cell. Neurite (murine superior cervical ganglia) and RBL cell [expressing the granule-associated antigen CD63-green fluorescent protein (GFP) conjugate] cocultures were established and stimulated with bradykinin (BK; 10 nM) or scorpion venom (SV; 10 pg/ml), both of which activate only neurites. Cell activation was assessed by confocal imaging of Ca2+ (cells preloaded with fluo 3), and analyses of RBL CD63-GFP+ granule movement were conducted. Neurite activation by BK or SV was followed by RBL Ca2+ mobilization, which was inhibited by an NK-1 receptor antagonist (NK-1 RA). Moreover, membrane ruffling was observed on RBL pseudopodial extensions in contact with the activated neurite, but not on noncontacting pseudopodia. RBL membrane ruffling was inhibited by NK-1 RA, but not NK-2 RA, and was accompanied by a significant increase in granule movement (0.13 +/- 0.04 vs. 0.05 +/- 0.01 microm/s) that was most evident at the point of neurite contact: many of the granules moved toward the plasmalemma. This is the first documentation of such precise (restricted to the membrane's contact site) transfer of information between nerves and mast cells that could allow for very subtle in vivo communication between these two cell types.