Comparative proteomic analysis provides insight into the key proteins as potential targets underlying the effect of malachite green against Ichthyophthirius multifiliis
Comparative proteomic analysis provides insight into the key proteins as potential targets underlying the effect of malachite green against Ichthyophthirius multifiliis
复制标题
比较蛋白质组学分析提供了对关键蛋白质的深入了解，这些蛋白质是孔雀石绿对抗多子鱼霉作用的潜在靶标
DOI：
10.1111/jfd.13346
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发表时间：
2021-02-09
影响因子：
2.5
通讯作者：
Ling, Fei
中科院分区：
文献类型：
--
作者：
Qu, Shenye;Song, Chenguang;Ling, Fei
靶点识别对于药物发现非常重要。不幸的是，迄今为止尚未在多丝小瓜虫中发现药物靶点，进一步限制了治疗小瓜虫病的新药的开发。在这项研究中，基于iTRAQ的定量蛋白质组学分析被用来寻找孔雀石绿色（MG）的目标，表现出比现有药物更大的效力，对I。多丝滋养体在原位。我们还通过RT-qPCR、TEM和细胞凋亡分析验证了蛋白质组学结果。我们的研究结果表明，在许多核糖体蛋白中发现了蛋白质丰度的主要变化，表明核糖体可能是一个候选靶标。GO和KEGG通路分析显示，在不同表达蛋白中，核糖体和PI 3 K-Akt信号通路明显富集。总之，上述DEP也通过RT-qPCR和形态学观察进行了验证。本研究为深入了解MG杀伤I的潜在靶点，揭示PI 3 K-Akt信号通路和核糖体通路中富集的关键蛋白。multifiliis，它可以作为其他毒性较小的药物的靶点，并作为I.多丝的
Target identification is important for drug discovery. Unfortunately, no drug targets have been found in Ichthyophthirius multifiliis until now and further limited development of the novel drug for Ichthyophthiriasis. In this study, an iTRAQ-based quantitative proteomic analysis was used to find the target of malachite green (MG), exhibiting greater efficacy than the existing drugs, against I. multifiliis trophonts in situ. We also verified the proteomic results by RT-qPCR, TEM and cell apoptosis assay. Our results showed that major variations in protein abundance were found among many of the ribosome proteins, indicating ribosome might be a candidate target. Furthermore, GO and KEGG pathway analyses of differentially expressed proteins (DEPs) revealed that ribosome and PI3K-Akt signalling pathway were remarkably enriched. Taken together, the above DEPs were also verified by RT-qPCR and morphological observations. This study provides insights into the key proteins enriched in PI3K-Akt signal pathway and ribosome pathway as potential targets of MG killing I. multifiliis, which could be served as targets for other less toxic drugs and be tested as potential treatments for I. multifiliis.