Response of Subgingival Bacteria to Smoking Cessation
Response of Subgingival Bacteria to Smoking Cessation
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DOI：
10.1128/jcm.01821-09
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发表时间：
2010-07-01
影响因子：
9.4
通讯作者：
Kumar, Purnima S.
中科院分区：
文献类型：
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作者：
Delima, Suzanne L.;McBride, Robert K.;Kumar, Purnima S.
已经证明，戒烟会改变龈下微生物的分布;然而，在这个生态系统中，单个细菌的反应还没有得到很好的研究。因此，本研究的目的是纵向研究戒烟对选定龈下细菌的患病率和水平的影响，使用分子方法进行细菌鉴定和计数。在基线和牙周非手术治疗和戒烟咨询后12个月，收集22名吸烟者的龈下菌斑。使用巢式PCR和基于多重珠粒的流式细胞术检查所选微生物的患病率和丰度。11名受试者在12个月内成功戒烟（戒烟者），而11名受试者继续吸烟（吸烟者）。戒烟导致12个月时牙髓卟啉单胞菌和肺炎小杆菌的患病率降低，微小微单胞菌、丝状菌和齿垢密螺旋体的水平降低。戒烟还导致小韦荣氏球菌水平的增加。在非手术牙周治疗和戒烟后，龈下微生物组被更多的健康相关物种所取代，并且推定的牙周病原体的患病率和丰度显着降低。结果表明，戒烟咨询在吸烟者牙周治疗中的关键作用是有效改变龈下微生物组。
It has been demonstrated that smoking cessation alters the subgingival microbial profile; however, the response of individual bacteria within this ecosystem has not been well studied. The aim of this investigation, therefore, was to longitudinally examine the effect of smoking cessation on the prevalence and levels of selected subgingival bacteria using molecular approaches for bacterial identification and enumeration. Subgingival plaque was collected from 22 smokers at the baseline and 12 months following periodontal nonsurgical management and smoking cessation counseling. The prevalence and abundance of selected organisms were examined using nested PCR and multiplexed bead-based flow cytometry. Eleven subjects successfully quit smoking over 12 months (quitters), while 11 continued to smoke throughout (smokers). Smoking cessation led to a decrease in the prevalence of Porphyromonas endodontalis and Dialister pneumosintes at 12 months and in the levels of Parvimonas micra, Filifactor alocis, and Treponema denticola. Smoking cessation also led to an increase in the levels of Veillonella parvula. Following nonsurgical periodontal therapy and smoking cessation, the subgingival microbiome is recolonized by a greater number of health-associated species and there are a significantly lower prevalence and abundance of putative periodontal pathogens. The results indicate a critical role for smoking cessation counseling in periodontal therapy for smokers in order to effectively alter the subgingival microbiome.