Temporal response of canine flexor tendon to limb suspension
Temporal response of canine flexor tendon to limb suspension
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DOI：
10.1152/japplphysiol.00051.2010
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发表时间：
2010-12-01
影响因子：
3.3
通讯作者：
Amadio, Peter C.
中科院分区：
文献类型：
--
作者：
Sun, Yu-Long;Thoreson, Andrew R.;Amadio, Peter C.
孙勇，索瑞森，查思生，赵超，安康，Amadio PC。犬屈肌腱对肢体悬吊的时间反应。中国生物医学工程学报（英文版），2009；首次发表于2010年10月14日；doi: 10.1152 / japplphysiol.00051.2010。-肌腱失用或应力剥夺常伴随临床疾病和治疗，但应力剥夺对肌腱代谢的影响很少有系统的研究。本文研究了应力剥夺对犬屈肌腱的影响。一只成年犬的前爪被暂停21天或42天。从没有对四肢和爪子进行干预的狗身上收集对照前爪。第21天，ⅰ、ⅲ型胶原的表达无显著变化，第42天，ⅰ、ⅲ型胶原的表达显著降低（P < 0.03）。悬吊肢肌腱中II型胶原、聚集蛋白、decorin和纤维连接蛋白的表达在第21天显著降低（P < 0.002），在第42天进一步降低。胁迫剥夺组在21和42 d时基质金属蛋白酶2 （MMP2）表达量显著升高（P < 0.004）。MMP3的表达在第21天和第42天显著降低（P < 0.03）。应激剥夺21和42 d时，MMP13的表达没有改变。MMP14的表达在第21天显著升高（P = 0.0015），在第42天恢复到对照水平。组织金属蛋白酶抑制剂1 （TIMP1）表达在悬吊42 d后下降（P = 0.0043）， 21 d后无明显变化。然而，TIMP2在21天和42天的表达与对照组没有显著差异。42 d时失应力肌腱的横截面积与对照组相比减小（P < 0.01）。本研究的干预方法未导致肌腱刚度的任何改变。我们的研究表明，应力剥夺减少了屈肌腱细胞外基质的合成代谢过程，增加了分解代谢过程。
Sun Y, Thoreson AR, Cha SS, Zhao C, An K, Amadio PC. Temporal response of canine flexor tendon to limb suspension. J Appl Physiol 109: 1762-1768, 2010. First published October 14, 2010; doi: 10.1152/japplphysiol.00051.2010.-Tendon disuse, or stress deprivation, frequently accompanies clinical disorders and treatments, yet the metabolism of tendons subject to stress deprivation has rarely been investigated systematically. The effects of stress deprivation on canine flexor tendon were investigated in this study. One adult canine forepaw was suspended for 21 or 42 days. Control forepaws were collected from dogs that had no intervention on their limbs and paws. The expression of collagen I and III was not significantly altered in the tendons disused for 21 days but was significantly decreased at 42 days (P < 0.03). The expression of collagen II, aggrecan, decorin, and fibronectin was significantly decreased in the tendons in the suspended limbs at 21 days (P < 0.002) and further reduced at 42 days. With stress deprivation, the expression of matrix metalloproteinase 2 (MMP2) was significantly increased (P < 0.004) at 21 and 42 days. The expression of MMP3 was significantly decreased at 21 and 42 days (P < 0.03). The expression of MMP13 was not altered with stress deprivation at 21 and 42 days. The expression of MMP14 was significantly increased at 21 days (P = 0.0015) and returned to the control level at 42 days. Tissue inhibitor of metalloproteinase 1 (TIMP1) expression was decreased after the limbs were suspended for 42 days (P = 0.0043), but not 21 days. However, TIMP2 expression was not significantly different from control at 21 or 42 days. Furthermore, the cross-sectional area of the stress-deprived tendons at 42 days was decreased compared with the control group (P < 0.01). The intervention method in this study did not result in any alteration of stiffness of the tendon. Our study demonstrated that stress deprivation decreases the anabolic process and increases the catabolic process of extracellular matrix in flexor tendon.