Enhanced robustness in acetone-butanol-ethanol fermentation with engineered Clostridium beijerinckii overexpressing adhE2 and ctfAB.
Enhanced robustness in acetone-butanol-ethanol fermentation with engineered Clostridium beijerinckii overexpressing adhE2 and ctfAB.
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DOI：
10.1016/j.biortech.2017.07.043
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发表时间：
2017-11
影响因子：
11.4
通讯作者：
Congcong Lu;Le Yu;S. Varghese;Mingrui Yu;Shangtian Yang
中科院分区：
文献类型：
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作者：
Congcong Lu;Le Yu;S. Varghese;Mingrui Yu;Shangtian Yang
将拜氏梭菌CC 101工程化以过表达醛/醇脱氢酶（adhE 2）和CoA-转移酶（ctfAB）。比较了仅表达adhE 2（CC 101-SV 4）和同时表达adhE 2、ald 4和ctfAB（CC 101-SV 6）的工程菌株与亲本菌株之间的溶剂产生和酸同化。CC 101-SV 4显示出从葡萄糖早期产生丁醇，但在丁醇浓度为1.6g/L时停止预发酵。与CC 101相比，CC 101-SV 6从葡萄糖中产生更多的丁醇（约12 g/L），并且能够再同化更多的酸，这在所有研究的条件下防止了“酸崩溃”并增加了丁醇产量。CC 101-SV 6还显示出更好的利用甘蔗渣水解产物中存在的葡萄糖和木糖的能力，并产生9.4 g/L的溶剂（丙酮、丁醇和乙醇），而CC 101仅为2.6 g/L，证实了其稳健性和对水解产物抑制剂的更好耐受性。C.过表达adhE 2和ctfAB的Beijerinckil应该具有从木质纤维素生物质水解产物生产丁醇的良好潜力。
Clostridium beijerinckiiCC101 was engineered to overexpress aldehyde/alcohol dehydrogenase (adhE2) and CoA-transferase (ctfAB). Solvent production and acid assimilation were compared between the parental and engineered strains expressing onlyadhE2 (CC101-SV4) and expressingadhE2,aldandctfAB(CC101-SV6). CC101-SV4 showed an early butanol production from glucose but stopped pre-maturely at a low butanol concentration of ∼6 g/L. Compared to CC101, CC101-SV6 produced more butanol (∼12 g/L) from glucose and was able to re-assimilate more acids, which prevented “acid crash” and increased butanol production, under all conditions studied. CC101-SV6 also showed better ability in using glucose and xylose present in sugarcane bagasse hydrolysate, and produced 9.4 g/L solvents (acetone, butanol and ethanol) compared to only 2.6 g/L by CC101, confirming its robustness and better tolerance to hydrolysate inhibitors. The engineered strain ofC. beijerinckiioverexpressingadhE2 andctfAB should have good potential for producing butanol from lignocellulosic biomass hydrolysates.