Correlation of plasma and tissue oxidative stresses in intra-abdominal sepsis
Correlation of plasma and tissue oxidative stresses in intra-abdominal sepsis
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DOI：
10.1016/j.jss.2004.07.246
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发表时间：
2004-12-01
影响因子：
2.2
通讯作者：
Uzun, H
中科院分区：
文献类型：
--
作者：
Koksal, GM;Sayilgan, C;Uzun, H
背景本研究的目的是探讨血浆和组织的氧化应激和抗氧化反应之间的相关性，通过测定丙二醛（AIDA）和谷胱甘肽（GSH）在盲肠结扎和穿孔诱导的晚期脓毒症。采用大鼠前瞻性随机对照实验研究。将40只体重200-250 g的大鼠随机分为两组（n = 20）。第1组在无菌条件下进行剖腹手术，结扎盲肠并穿孔。腹部闭合。假手术对照组（2组）仅行开腹手术。24 h后，心脏穿刺取血测定血浆丙二醛（MDA）和谷胱甘肽（GSH）水平，同时取肺、肝、肾和心脏组织标本。在肝、肺、血浆、心脏和肾脏中，24小时后脓毒症组的MDA浓度增加（所有器官样品P < 0.001）。在相同的器官中，GSH浓度降低脓毒症（P < 0.001的所有器官样本）。两组血浆MDA与心（r = 0.82，P < 0.001）、肝（r = 0.76，P <0.001）、肺（r = 0.78，P <0.001）、肾（r = 0.73，P < 0.001）MDA均呈正相关。血浆GSH与肝（r = 0.939，P < 0.001）、心（r = 0.86，P < 0.001）、肺（r = 0.91，P <0.001）、肾（r = 0.79，P < 0.001）GSH均呈正相关。腹腔脓毒症大鼠血浆MDA、GSH与组织MDA、GSH呈正相关。(C)2004年爱思唯尔公司All rights reserved.
Background. The aim of this study was to investigate the correlation between plasma and tissue oxidative stress and the antioxidative response, by measuring malon dialdehyde (AIDA) and glutathione (GSH) in late sepsis induced by cecal ligation and perforation.Materials and methods. A prospective, randomized, controlled experimental study in rats was done. Forty rats, weighing 200-250 g, were randomly divided into two groups (n = 20). In group 1, laparotomy was performed under aseptic conditions, and the cecum ligated and perforated. The abdomen was closed. In group 2, sham control, there was only laparotomy. Twenty-four hours later, blood samples were taken by cardiac puncture for plasma MDA and GSH, followed by harvesting of samples from lung, liver, kidney, and heart in both groups.Results. In the liver, lung, plasma, heart, and kidney, MDA concentrations were increased in the sepsis group after 24 h (P < 0.001 for all organ samples). In the same organs, GSH concentrations were decreased by sepsis (P < 0.001 for all organ samples). In both groups, plasma MDA was positively correlated to MDA in heart (r = 0.82, P < 0.001), liver (r = 0.76, P < 0.001), lung (r = 0.78, P < 0.001), and kidney (r = 0.73, P < 0.001). Similarly, plasma GSH was positively correlated to GSH in liver (r = 0.939 P < 0.001), heart (r = 0.86, P < 0.001), lung (r = 0.91, P < 0.001), and kidney (r = 0.79, P < 0.001).Conclusions. Plasma MDA and GSH were positively correlated with tissue MDA and GSH in intra-abdominal sepsis in a rat model. (C) 2004 Elsevier Inc. All rights reserved.