Chlorophyll a′ and pheophytin a, as determined by HPLC, in photosynthesis mutants and double mutants of Chlamydomonas reinhardtii
Chlorophyll a′ and pheophytin a, as determined by HPLC, in photosynthesis mutants and double mutants of Chlamydomonas reinhardtii
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HPLC 测定莱茵衣藻光合作用突变体和双突变体中的叶绿素 a 和脱镁叶绿素 a
DOI：
10.1016/0005-2728(90)90111-g
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发表时间：
1990
期刊：
影响因子：
--
通讯作者：
A. Trémolières
中科院分区：
文献类型：
--
作者：
J. Maroc;A. Trémolières
用高效液相色谱法（HPLC）分析了莱氏披衣（Chlamydiumreinhardtii）野生型和7个光合功能受损突变体的叶绿素（Chl）a′和脱镁叶绿素（Pheo）含量。其中4个没有或很少的光系统II（PS II）的活动。两个没有光系统I（PS I）的活动，没有P-700。在第七个突变体中，两个光系统都存在，但主要的捕光天线（对应于PS II的叶绿素蛋白复合物：CP II）是完全缺乏，因为没有叶绿素b。在PS II活性受损的突变体中，Pheoai缺乏或仅少量存在，与PS II活性的丧失直接相关。这些结果与Pheoaa在PS II中作为初级电子受体的众所周知的作用一致。在PS Ⅱ活性受损的突变体中，Chla′水平是正常的。在PS I活性受损的突变体中（P-700较少的突变体），Chla′的含量只有正常含量的一半。因此，Chla′仅与PS I相关，如Watanabe（Watanabe，T.，小林，M.，Hongu，A.，Nakazato，M.，Hiyama，T.和Murata，N. 04 The Dog of the Woman（1985）191，252-256）。但只有一半的Chla′含量可能是P-700。最后一个结果与Watanabe关于整个Chla′构成P-700的结论相矛盾。另一方面，Chla′的存在与PS I：CP 0a的第二个Chla蛋白复合物之间没有联系。讨论了Chla′与PS Ⅰ活性相关的可能功能。
HPLC analysis of chlorophyll (Chl)a′ and pheophytin (Pheo)acontent was run for wild typeChlamydomonas reinhardtiiand seven mutants with impaired photosynthetic functions. Four of them had no or little Photosystem II (PS II) activity. Two had no Photosystem I (PS I) activity and no P-700. In the seventh mutant, both photosystems were present but the main light-harvesting antenna (the chlorophyll-protein complex corresponding to PS II: CP II) was totally lacking because of the absence of Chlb. In mutants impaired in PS II activity, Pheoais either lacking or found in only low amounts in direct relation with the loss in PS II activity. These results are in agreement with the well-known role of Pheoaas a primary electron acceptor in PS II. In mutants impaired in PS II activity the Chla′ level is normal. In mutants impaired in PS I activity (P-700 less mutants), only half of the normal content of Chla′ is present. So Chla′ is associated exclusively with PS I as demonstrated before by Watanabe (Watanabe, T., Kobayashi, M., Hongu, A., Nakazato, M., Hiyama, T. and Murata, N. (1985) FEBS Lett. 191, 252–256). But only half the Chla′ content could be P-700. This last result contradicts Watanabe's conclusion that the whole Chla′ constitutes P-700. On the other hand, there is no relation between the presence of Chla′ and the second Chla-protein complex of PS I: CP 0a. The possible function of Chla′ related to PS I activity is discussed.