Chemo-microbial conversion of cellulose into polyhydroxybutyrate through ruthenium-catalyzed hydrolysis of cellulose into glucose.
Chemo-microbial conversion of cellulose into polyhydroxybutyrate through ruthenium-catalyzed hydrolysis of cellulose into glucose.
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通过钌催化纤维素水解成葡萄糖，将纤维素化学微生物转化为聚羟基丁酸酯。
DOI：
10.1016/j.biortech.2010.09.098
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发表时间：
2011
影响因子：
11.4
通讯作者：
S. Taguchi
中科院分区：
文献类型：
--
作者：
Ken’ichiro Matsumoto;Hirokazu Kobayashi;K. Ikeda;T. Komanoya;A. Fukuoka;S. Taguchi
使用负载型钌催化剂产生的纤维素衍生的葡萄糖被应用于重组大肠杆菌中的聚（3-羟基丁酸酯）[P(3HB)]生产。通过在220℃下与催化剂反应，15-20碳摩尔％的纤维素转化为葡萄糖。水解产物还含有副产物，例如果糖、甘露糖、左旋葡聚糖、低聚纤维素、5-羟甲基糠醛(5-HMF)和糠醛以及未鉴定的化合物。设置较低的反应温度（215°C）可以提高葡萄糖与5-HMF的比例，这是细胞生长的主要抑制因素。事实上，重组大肠杆菌对215°C产生的水解产物表现出更好的性能，并且累积P(3HB)高达42wt%，这与相同浓度的分析级葡萄糖的情况相同。结果表明，钌介导的纤维素水解具有作为从纤维素生物质生产生物基塑料的有用生物精炼工艺的效力。
Cellulose-derived glucose generated using the supported ruthenium catalyst was applied to poly(3-hydroxybutyrate) [P(3HB)] production in recombinant Escherichia coli. By the reaction with the catalyst at 220°C, 15–20 carbon mol% of cellulose was converted into glucose. The hydrolysate also contained byproducts such as fructose, mannose, levoglucosan, oligomeric cellulose, 5-hydroxymethylfurfural (5-HMF), and furfural together with unidentified compounds. Setting the reaction temperature lower (215°C) improved the ratio of glucose to 5-HMF, which was a main inhibiting factor for the cell growth. Indeed, the recombinant E. coli exhibited better performance on the hydrolysate generated at 215°C and accumulated P(3HB) up to 42wt%, which was the same as the case of the same concentration of analytical grade glucose. The result indicated that the ruthenium-mediated cellulose hydrolysis has a potency as a useful biorefinery process for production of bio-based plastic from cellulosic biomass.