Chapter 3. In-cell NMR in Eukaryotic Cells I (Introduction from Outside of Cells)
Chapter 3. In-cell NMR in Eukaryotic Cells I (Introduction from Outside of Cells)
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第3章真核细胞内核磁共振I（细胞外介绍）
DOI：
10.1039/9781788013079-00027
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发表时间：
2019
期刊：
影响因子：
--
通讯作者：
Shirakawa Masahiro
中科院分区：
文献类型：
--
作者：
Inomata Kohsuke;Kigawa Takanori;Shirakawa Masahiro
细胞内NMR光谱学的历史始于大肠杆菌细胞中同位素标记蛋白质的高分辨率多维NMR光谱学1，这是因为成熟的样品制备方法，例如目标蛋白质的选择性过表达和稳定同位素标记。实际上，利用E.在首次报道细胞内NMR实验后，提出了大肠杆菌细胞。2-7然而，有限的细胞类型的使用仅在生物大分子特别是蛋白质的“原位”观察方面具有有限的应用，这意味着只有E.大肠杆菌蛋白应在大肠杆菌中进行研究。大肠杆菌细胞。不像
The history of in-cell NMR spectroscopy started as the high-resolution multidimensional NMR spectroscopy of isotope-labelled proteins in Escherichia coli cells1 because of well-established sample preparation methods such as selective overexpression and stable isotope labelling of the target proteins. Indeed, various applications utilizing E. coli cells were proposed after the first report of in-cell NMR experiments. 2–7 However, the limited employment of cell types has only limited applications in terms of ‘‘in situ’’observation of biological macromolecules especially proteins, meaning that only E. coli proteins should be investigated in E. coli cells. Unlike the case of