Efficient arachidonic acid-rich oil production by Mortierella alpina through a three-stage fermentation strategy
Efficient arachidonic acid-rich oil production by Mortierella alpina through a three-stage fermentation strategy
复制标题
高山被孢霉通过三阶段发酵策略高效生产富含花生四烯酸的油
DOI：
10.1007/s00449-013-1015-2
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发表时间：
2014-03-01
影响因子：
3.8
通讯作者：
Huang, He
中科院分区：
文献类型：
--
作者：
Nie, Zhi-Kui;Deng, Zhong-Tao;Huang, He
以高山被孢霉为研究对象，设计了一种高效生产富含花生四烯酸油脂的三级发酵工艺。通过改变培养基的组成，对不同阶段的工艺进行了研究。在第一阶段，菌丝体接种在营养丰富的培养基中进行快速繁殖。在第二阶段，收集菌丝体，然后在葡萄糖溶液中培养以获得高细胞脂质含量。在第三阶段，菌丝体在无葡萄糖培养基中培养以获得快速的ARA积累。采用这种发酵策略，高干细胞重量，脂质和ARA浓度分别达到41.6，26.6和11.4 g/L。结果表明，菌丝体繁殖，脂质生物合成和ARA积累过程可以在空间上显著分离，允许进一步优化以提高每个阶段的效率。这是第一次使用三阶段发酵策略生产富含ARA的油脂，它可以应用于其他类似的产油微生物，以获得高相关的多不饱和脂肪酸积累。
A three-stage fermentation strategy was designed for efficient arachidonic acid (ARA)-rich oil production by Mortierella alpina. The process at different stages by changing the components of medium was investigated. In the first stage, mycelia were inoculated in a nutrient-rich medium for rapid propagation. In the second stage, mycelia were collected and then cultivated in glucose solution to achieve high cellular lipid contents. In the third stage, mycelia were cultured in a glucose-absent medium to obtain rapid ARA accumulation. Using this fermentation strategy, high dry cell weight, lipid, and ARA concentration reached 41.6, 26.6, and 11.4 g/L, respectively. The results demonstrated that mycelia propagation, lipid biosynthesis, and ARA accumulation process can be significantly spatially separated, allowing further optimization to improve the efficiency of each stage. This was the first report of using a three-stage fermentation strategy for ARA-rich oil production, and it could be applied to other similar oleaginous microorganisms to obtain high related polyunsaturated fatty acids accumulation.