Sensitive NMR sensors detect antibodies to influenza
Sensitive NMR sensors detect antibodies to influenza
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DOI：
10.1002/anie.200800069
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发表时间：
2008-01-01
影响因子：
16.6
通讯作者：
Josephson, Lee
中科院分区：
文献类型：
--
作者：
Koh, Isaac;Hong, Rid;Josephson, Lee
用于确定免疫状态的医疗点（POC）传感器是防备禽流感等可能造成的大流行病的重要组成部分。[1,2]基于核磁共振的磁松弛开关（MRSw）在这一应用中很有吸引力，因为它们对光不敏感，并且不涉及在血管壁上固定材料磁共振弛豫仪作为POC传感器是实用的，因为它们对磁场强度、体积和均匀性的要求最小。[4-6]迄今为止，MRSw已经使用磁性纳米颗粒（NPs）与分子靶标反应来聚集和降低T2。虽然MRSw检测高价病毒或细菌具有高灵敏度[7,8]，但它们对蛋白质的敏感性要低得多。[9,10]我们的目标是提高MRSw传感器对二价抗体的灵敏度，因此鸟类或人类的免疫状态最终可能会使用POC MR松弛仪来确定。一种识别人流感病毒血凝素标签肽的单克隆抗体被用作评估提高MRSw敏感性策略的靶标。
Point of care (POC) sensors for the determination of immune status are an important part of the preparation for possible pandemics such as that created by avian influenza.[1, 2] NMR based magnetic relaxation switches (MRSw's) are attractive for this application because they are indifferent to light and involve no immobilization of materials on vessel walls.[3] MR relaxometers are practical as POC sensors since their requirements for magnetic field strength, volume and homogeneity are minimal.[4-6]To date, MRSw's have used magnetic nanoparticles (NPs) that react with molecular targets to aggregate and decrease T2. Although MRSw's detect highly multivalent viruses or bacteria with high sensitivity,[7, 8] their sensitivity for proteins is far lower.[9, 10] Our goal was to improve the sensitivity of MRSw sensors for divalent antibodies, so the immune status of birds or humans might eventually be determined using a POC MR relaxometer. A monoclonal antibody recognizing the Tag peptide from a hemagglutinin of a human influenza virus was used as a target to assess strategies for improving MRSw sensitivity.