Enzymatic amplification of specific cDNA inserts from λgt11 libraries
Enzymatic amplification of specific cDNA inserts from λgt11 libraries
复制标题
对 λgt11 文库中的特定 cDNA 插入片段进行酶促扩增
DOI：
10.1093/nar/16.17.8718
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发表时间：
1988
影响因子：
14.9
通讯作者：
R. Montgomery
中科院分区：
文献类型：
--
作者：
K. Friedman;N. L. Rosen;P. Newman;R. Montgomery
从噬菌体文库中分离特异性cDNA是一个耗时的过程，包括筛选、阳性克隆的噬斑纯化、λ DNA制备和将相关插入片段亚克隆到质粒中。最近已经证明，来自单个噬菌体（1）或质粒（2）克隆的cDNA插入物可以使用聚合酶链式反应（PCR）酶促扩增。我们已经修改了这一技术，以获得特定的目标cDNA直接从Agt11库。
Isolation of specific cDNA's from bacteriophage libraries is a time consuming process, involving screening, plaque purification of positive clones, lambda DNA preparation, and subcloning of relevant inserts into plasmid. It has recently been demonstrated that cDNA inserts from individual bacteriophage (1) or plasmid (2) clones can be enzymatically amplified using the polymerase chain reaction (PCR). We have modified this technique in order to obtain specific target cDNA directly from a Agtl 1 library.