Microcystin-LR triggers different endoplasmic reticulum stress pathways in the liver, ovary, and offspring of zebrafish (Danio rerio)
Microcystin-LR triggers different endoplasmic reticulum stress pathways in the liver, ovary, and offspring of zebrafish (Danio rerio)
复制标题
微囊藻毒素-LR 在斑马鱼 (Danio rerio) 的肝脏、卵巢和后代中触发不同的内质网应激途径
DOI：
10.1016/j.jhazmat.2019.121939
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发表时间：
2020
影响因子：
13.6
通讯作者：
Xuezhen Zhang
中科院分区：
文献类型：
--
作者：
Chunhua Zhan;Wanjing Liu;Feng Zhang;Xuezhen Zhang
The existence of microcystins (MCs), the secondary metabolite of cyanobacteria, has become a growing public health concern. Previous researches have proved that MCs can trigger endoplasmic reticulum stress (ERS), but the underlying mechanisms remain unclear. In the present study, adult female zebrafish were exposed to MC-LR (0, 1, 5 and 20 μg/L) for 30 d, and the offspring derived from the treated females and healthy males were cultured in water without MC-LR until 96 h post fertilization (hpf). Our data suggested that MC-LR causes a significant increase in theeif2s1a,atf4, andeif2ak3transcription levels in the liver and ovary. The mRNA levels ofatf4,atf6,bcl-2,hspa5,eif2s1aandeif2ak3upregulated notably in the offspring. JNK phosphorylation level and cleaved-caspase3 protein expression elevated obviously in the liver and ovary, but had no remarkable change in the offspring. Furthermore, TUNEL results showed that MC-LR significantly induced apoptosis in the liver and ovary, while acridine orange (AO) staining indicated that MC-LR did not cause abnormal apoptosis in offspring. Concisely, the present study indicated that MC-LR leads to apoptosis through different ERS pathways in the liver, ovary and offspring, and also provides a new perspective for understanding the apoptosis caused by MC-LR.
