Dpp Signaling Activity Requires Pentagone to Scale with Tissue Size in the Growing Drosophila Wing Imaginal Disc
Dpp Signaling Activity Requires Pentagone to Scale with Tissue Size in the Growing Drosophila Wing Imaginal Disc
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DOI：
10.1371/journal.pbio.1001182
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发表时间：
2011-10-01
期刊：
影响因子：
9.8
通讯作者：
Affolter, Markus
中科院分区：
文献类型：
--
作者：
Hamaratoglu, Fisun;de Lachapelle, Aitana Morton;Affolter, Markus
果蝇（Drosophila melanogaster）的翅膀具有简单的二维结构，是非常适合系统生物学方法的模型器官。翅膀由称为翅成虫盘的上皮囊产生，在幼虫阶段经历大量生长和伴随的图案形成阶段。十肢麻痹 (Dpp) 形态发生素在翅膀形成过程中发挥着核心作用，具有协调调节图案形成和生长的能力。在这里，我们询问 Dpp 信号传导活动是否随着翼成虫盘的生长而缩放，即成比例扩展。使用新方法对 Dpp 活性及其缩放特性进行空间和时间量化，我们发现 Dpp 响应随着生长组织的大小而缩放。值得注意的是，缩放并不是在该领域的所有位置都是完美的，并且目标基因域的缩放特别是在它们定义静脉位置的地方得到保证。我们还发现，目标基因域在下游 Dpp 活性梯度 P-Mad 和 Brinker 的恒定浓度阈值下没有定义。最有趣的是，Pentagon 是该通路的重要分泌反馈调节因子，在缩放过程中发挥着核心作用，并在椎间盘生长过程中充当 Dpp 梯度的扩展器。
The wing of the fruit fly, Drosophila melanogaster, with its simple, two-dimensional structure, is a model organ well suited for a systems biology approach. The wing arises from an epithelial sac referred to as the wing imaginal disc, which undergoes a phase of massive growth and concomitant patterning during larval stages. The Decapentaplegic (Dpp) morphogen plays a central role in wing formation with its ability to co-coordinately regulate patterning and growth. Here, we asked whether the Dpp signaling activity scales, i.e. expands proportionally, with the growing wing imaginal disc. Using new methods for spatial and temporal quantification of Dpp activity and its scaling properties, we found that the Dpp response scales with the size of the growing tissue. Notably, scaling is not perfect at all positions in the field and the scaling of target gene domains is ensured specifically where they define vein positions. We also found that the target gene domains are not defined at constant concentration thresholds of the downstream Dpp activity gradients P-Mad and Brinker. Most interestingly, Pentagone, an important secreted feedback regulator of the pathway, plays a central role in scaling and acts as an expander of the Dpp gradient during disc growth.