Role of rsbU and staphyloxanthin in phagocytosis and intracellular growth of Staphylococcus aureus in human macrophages and endothelial cells.
Role of rsbU and staphyloxanthin in phagocytosis and intracellular growth of Staphylococcus aureus in human macrophages and endothelial cells.
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rsbU 和葡萄球菌黄质在人巨噬细胞和内皮细胞中金黄色葡萄球菌的吞噬作用和细胞内生长中的作用。
DOI：
10.1086/606012
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发表时间：
2009
期刊：
影响因子：
--
通讯作者：
Oldfield,Eric
中科院分区：
文献类型：
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作者：
Olivier,AurélieC;Lemaire,Sandrine;VanBambeke,Françoise;Tulkens,PaulM;Oldfield,Eric
在金黄色葡萄球菌中，rsbU下调葡萄球菌素，刺激葡萄球菌黄素（STX）的产生，STX是一种抗氧化剂，可能有助于吞噬后的细胞内存活。使用同基因rsbU −和rsbU+菌株，我们发现rsbU导致THP-1巨噬细胞和人脐静脉内皮细胞（后者更是如此）的内化和细胞内生长增加，而不改变亚细胞定位，抑制STX生物合成显着降低了thersbU+菌株（和临床分离株，包括USA 300;仅用巨噬细胞测试）的细胞内生长，而不影响内化。因此，rsbU是重要的摄取和STX的生物合成，是至关重要的细胞内增殖的S。金黄色
InStaphylococcus aureus,rsbUdown-regulatesagrand stimulates production of staphyloxanthin (STX), an antioxidant that may contribute to intracellular survival after phagocytosis. Using isogenicrsbU−andrsbU+strains, we show thatrsbUcauses increased internalization and intracellular growth in both THP-1 macrophages and human umbilical vein endothelial cells (more so for the latter) without change in subcellular localization and that inhibition of STX biosynthesis markedly reduces intracellular growth of thersbU+strain (and of clinical isolates, including USA300; tested with macrophages only) without affecting internalization. Thus,rsbUis important for uptake and for STX biosynthesis and is critical for intracellular multiplication ofS. aureus