ROLE OF ENDOTHELIAL-CELLS IN RELAXATION OF ISOLATED ARTERIES BY BRADYKININ
ROLE OF ENDOTHELIAL-CELLS IN RELAXATION OF ISOLATED ARTERIES BY BRADYKININ
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DOI：
10.1073/pnas.79.6.2106
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发表时间：
1982-01-01
期刊：
影响因子：
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通讯作者：
JOTHIANANDAN, D
中科院分区：
文献类型：
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作者：
CHERRY, PD;FURCHGOTT, RF;JOTHIANANDAN, D
缓激肽引起犬冠状动脉、腹腔动脉、肠系膜上动脉、肾动脉、脾动脉、肺动脉、胃动脉和股动脉离体横环的松弛。通过摩擦内膜表面去除血管壁内皮细胞后，犬动脉在加入缓激肽后不能放松。用环氧化酶抑制剂（吲哚美辛和氟比洛芬）治疗后，犬动脉的内皮依赖性松弛保持完整，这与前列腺素的介导作用相反。当犬动脉内皮细胞受到缓激肽刺激时，它们会释放一种介导血管平滑肌松弛的物质。相反，猫（上肠系膜）和兔（上肠系膜和乳糜泻）的动脉制剂可以在内膜表面摩擦，而不会持续失去对缓激肽放松作用的敏感性。此外，猫和兔动脉的松弛被环氧化酶抑制剂完全阻断。缓激素以内皮依赖的方式使人动脉松弛，这种作用不是由前列腺素介导的。所有物种的动脉都需要内皮细胞的存在来放松对乙酰胆碱的反应，并且，使用兔子主动脉，这种效果被证明是通过从这些细胞释放一种未表征的物质来调节的，这种物质可以放松血管平滑肌。缓激素通过类似的机制放松犬和人的动脉，但它通过刺激前列腺素的释放来放松猫和兔子的动脉，但尚未明确的细胞类型。
Bradykinin elicits relaxation of isolated transverse rings of canine coronary, celiac, superior mesenteric, renal, splenic, pulmonary, gastric and femoral arteries. After endothelial cells of the vessel wall are removed by rubbing of the intimal surface, canine arteries fail to relax upon addition of bradykinin. The endothelium-dependent relaxation of canine arteries remains intact after treatment with cyclooxygenase inhibitors (indomethacin and flurbiprofen), and this argues against mediation by prostaglandins. When they are stimulated with bradykinin, endothelial cells of canine arteries appear to release a substance mediating vascular smooth muscle relaxation. In contrast, preparations of arteries of cats (superior mesenteric) and rabbits (superior mesenteric and celiac) may be rubbed on the intimal surface without a consistent loss of sensitivity to the relaxing effects of bradykinin. In addition, relaxation of the cat and rabbit arteries is completely blocked by cyclooxygenase inhibitors. Bradykinin relaxes human arteries in an endothelium-dependent manner and this effect is not mediated by prostaglandins. Arteries of all species tested require the presence of endothelial cells for relaxation in response to acetylcholine and, using the rabbit aorta, this effect was shown to be mediated by the release of an uncharacterized substance from these cells that relaxes vascular smooth muscle. Bradykinin relaxes canine and human arteries via a similar mechanism but it relaxes cat and rabbit arteries by stimulating release of prostaglandins from as yet undefined cell types.