Graphene oxide regulates the bacterial community and exhibits property changes in soil
Graphene oxide regulates the bacterial community and exhibits property changes in soil
复制标题
氧化石墨烯调节细菌群落并表现出土壤特性变化
DOI：
10.1039/c5ra01045d
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发表时间：
2015-01-01
期刊：
影响因子：
3.9
通讯作者：
Zhou, Qixing
中科院分区：
文献类型：
--
作者：
Du, Junjie;Hu, Xiangang;Zhou, Qixing
原生氧化石墨烯（PGO）的广泛使用增加了其环境释放。PGO与土壤（排放纳米材料的最终储存库之一）的相互作用仍不清楚。在本研究中，基于细菌16 S rRNA基因的焦磷酸测序分析表明，细菌群落中的PGO土壤样品（PGOS）变得更丰富，更多样性与对照土壤样品（CS）相比。PGO改变了土壤细菌群落结构，一些固氮和异化铁还原细菌被选择性富集，特别是在属水平上。与PGO相比，SGO（土壤改性氧化石墨烯）具有更大的厚度、更高的C/O比、更粗糙的纹理、更低的透明度和更小的尺寸。SGO中含有含氮基团和Mg、Al、Si、K、Ca、Fe等元素。表面基团的变化与有机分子包覆SGO的形成一致。SGO具有较少的负电荷，比PGO更不稳定。此外，SGO的化学活性高于PGO，例如，SGO具有更多的未成对电子和无序结构。这项工作突出了PGO和土壤的关键相互作用，在评估纳米材料的风险时值得全面考虑。
The extensive use of pristine graphene oxide (PGO) increases its environmental release. The interactions of PGO with soils, one of the ultimate repositories for discharged nanomaterial, remain unclear. In the present study, a pyrosequencing analysis based on the bacterial 16S rRNA gene showed that the bacterial community in a PGO-soil sample (PGOS) became richer and more diverse compared with a control soil sample (CS). PGO altered the structure of soil bacterial communities, with some nitrogen-fixing and dissimilatory iron reducing bacteria being selectively enriched, especially at the genus level. SGO (soil-modified graphene oxide) exhibited a greater thickness, a higher C/O ratio, a rougher texture, a lower transparency and a smaller size than PGO. Nitrogen-containing groups and the elements including Mg, Al, Si, K, Ca and Fe were detected in SGO. The changes in surface groups were consistent with the formation of organic molecules coating the SGO. SGO, which exhibited fewer negative charges, was more unstable than PGO. In addition, SGO presented higher chemical activity than PGO; for example, SGO exhibited more unpaired electrons and disordered structures. This work highlights the critical interactions of PGO and soil which deserve comprehensive consideration in assessing the risks of nanomaterials.