Activation of the TREM-1 pathway in human monocytes by periodontal pathogens and oral commensal bacteria.
Activation of the TREM-1 pathway in human monocytes by periodontal pathogens and oral commensal bacteria.
复制标题
通过牙周病原体和口服共生细菌激活人单核细胞中的TREM-1途径。
DOI：
10.1111/omi.12169
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发表时间：
2017-08
影响因子：
3.7
通讯作者：
Scannapieco FA
中科院分区：
文献类型：
--
作者：
Varanat M;Haase EM;Kay JG;Scannapieco FA
牙周炎是一种高度流行的疾病，部分原因是宿主对口腔多物种生物膜的异常反应。口腔细菌和宿主免疫力之间的平衡对口腔健康至关重要。口腔微生物组的不平衡导致不受控制的宿主炎症反应和随后的牙周病（即牙龈炎和牙周炎）。TREM-1是存在于骨髓细胞上的信号传导受体，能够与其他模式识别受体协同作用，导致炎症反应的放大。本研究的目的是研究TREM-1途径在人类单核细胞样细胞系THP-1暴露于口腔病原体和口腔粘膜的激活。通过实时定量聚合酶链反应测定编码TREM-1及其衔接蛋白DAP 12的基因的相对表达。通过流式细胞术测定TREM-1的表面表达。通过酶联免疫吸附测定法测量可溶性TREM-1和细胞因子。结果表明，口腔细菌和致病性口腔细菌都激活TREM-1途径，导致促炎性细胞因子产生的促炎性TREM-1活性依赖性增加。
Periodontitis is a highly prevalent disease caused in part by an aberrant host response to the oral multi-species biofilm. A balance between the oral bacteria and host immunity is essential for oral health. Imbalances in the oral microbiome lead to an uncontrolled host inflammatory response and subsequent periodontal disease (i.e. gingivitis and periodontitis). TREM-1 is a signaling receptor present on myeloid cells capable of acting synergistically with other pattern recognition receptors leading to amplification of inflammatory responses. The aim of this study was to investigate the activation of the TREM-1 pathway in the human monocyte-like cell line THP-1 exposed to both oral pathogens and commensals. The relative expression of the genes encoding TREM-1 and its adapter protein DAP12 were determined by quantitative real-time polymerase chain reaction. The surface expression of TREM-1 was determined by flow cytometry. Soluble TREM-1 and cytokines were measured by enzyme-linked immunosorbent assay. The results demonstrate that both commensal and pathogenic oral bacteria activate the TREM-1 pathway, resulting in a proinflammatory TREM-1 activity-dependent increase in proinflammatory cytokine production.