Conserved elements in the 5' regulatory region of the amyloid precursor protein gene in primates
Conserved elements in the 5' regulatory region of the amyloid precursor protein gene in primates
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DOI：
10.1016/s0304-3940(97)00283-8
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发表时间：
1997-05-02
影响因子：
2.5
通讯作者：
Oliva, R
中科院分区：
文献类型：
--
作者：
Adroer, R;LopezAcedo, C;Oliva, R
利用人和小鼠淀粉样前体蛋白(APP)基因之间保守区域的寡核苷酸，对黑猩猩、大猩猩、猩猩、木瓜和非洲绿猴的APP基因启动子区域进行聚合酶链式反应(PCR)扩增和测序。APP基因启动子序列比对后发现了几个新的保守的潜在调控序列：-450位的载脂蛋白E-B1(APOE-B1)元件，-450位和-301位的两个激活蛋白-2(AP-2)位，以及-280位的中间早期1基因(IE1)。这些元件在所有研究的哺乳动物APP启动子序列中都是保守的。此外，先前检测到的-317位的热休克元件(HSE)和-350位的激活蛋白-1(AP-1)也是保守的。了解APP基因的基本调控元件是理解其转录调控和后续模型研究的基础。(C)1997年爱思唯尔爱尔兰科学有限公司。
Oligonucleotides corresponding to conserved sites between the human and mouse amyloid precursor protein (APP) genes have been used to polymerase chain reaction (PCR) amplify and sequence the promoter region of the APP gene from chimpanzee, gorilla, orang-utan, papio and African green monkey. Several novel conserved potentially-regulatory sequences of the APP gene have been detected after alignment of the APP promoter sequences: an apolipoprotein E-B1 (APOE-B1) element at position -450, also present in the APOE gene, two activator protein-2 (AP-2) sites at positions -450 and -301 and an intermediate early-1 gene (IE1) site at position -280. These elements are conserved in all mammalian APP promoter sequences studied. Additionally a previously detected heat shock element (HSE) at position -317, and an activator protein-1 (AP-1) site at position -350 are also conserved. Knowledge of the essential regulatory elements at the APP gene constitute the basis for understanding its transcriptional control and subsequent model studies. (C) 1997 Elsevier Science Ireland Ltd.