Prevalence of Severe Fever with Thrombocytopenia Syndrome Virus in Ticks Collected from National Parks in Korea
Prevalence of Severe Fever with Thrombocytopenia Syndrome Virus in Ticks Collected from National Parks in Korea
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DOI：
10.1089/vbz.2018.2338
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发表时间：
2018-11-27
影响因子：
2.1
通讯作者：
Chae, Joon-Seok
中科院分区：
文献类型：
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作者：
Jo, Young-Sun;Kang, Jun-Gu;Chae, Joon-Seok
重度发热伴血小板减少综合征（SFTS）是东亚国家（包括中国、日本和韩国）的一种新兴病毒性疾病。SFTS的病原体是由蜱传播的SFTS病毒（SFTSV）。为了调查SFTSV在韩国的流行情况，2015年7月至11月期间，从五个国家公园的植被中共收集了9744只蜱。在所采集的成蜱和若蜱中，长角血蜱的数量最多（68.44%），其次是黄血蜱（29.66%）、日本硬蜱（1.56%）和龟形钝眼蜱（0.34%）。所采集的蜱幼虫为血蜱属（99.61%）和硬蜱属（0.39%）。采用一步法RT-PCR和巢式PCR检测了SFTSV在各蜱虫成虫、若虫和混合蜱虫幼虫中的特异性基因。H. SFTSV的检出率为4.77%（48/1006）。longicornis，1.15%（5/436）;黄曲霉（flava）为0%（0/23）; nipponensis，A.龟鳖馆成蜱和若蜱中SFTSV的感染率为3.61%。与以往报道相比，SFTSV在成蜱和若虫蜱中的流行率相对较高。蜱幼虫的最低感染率为0.31%。在国家公园的步道和非步道区域收集的蜱虫中检测到SFTSV，海拔高达800米。所获得的序列与中国和日本蜱SFTS病毒S片段序列的同源性为99.4-99.7%。
Severe fever with thrombocytopenia syndrome (SFTS) is an emerging viral disease in East Asian countries, including China, Japan, and the Republic of Korea (ROK). The causative agent of SFTS is the SFTS virus (SFTSV), which is transmitted by ticks. To investigate the prevalence of SFTSV in the ROK, a total of 9744 ticks were collected from vegetation in five national parks between July and November 2015. Of the collected adult and nymph ticks, Haemaphysalis longicornis (68.44%) was the most abundant, followed by Haemaphysalis flava (29.66%), Ixodes nipponensis (1.56%), and Amblyomma testudinarium (0.34%). Collected larval ticks were of the genera Haemaphysalis (99.61%) and Ixodes (0.39%). One-step RT-PCR and nested PCR were used to detect SFTSV-specific genes from each individual adult and nymph tick and pooled larval ticks. SFTSV was detected in 4.77% (48/1006) in H. longicornis, 1.15% (5/436) in H. flava, 0% (0/23) in I. nipponensis, and 20% (1/5) in A. testudinarium. The infection rate of SFTSV in adult and nymph ticks was 3.61%. The prevalence of SFTSV in adult and nymph ticks was relatively high, compared with previous reports. In larval ticks, the minimum infection rate was 0.31%. SFTSV was detected in ticks collected from both trail and nontrail areas in the national parks, and up to 800 meters above sea level. The sequences obtained showed 99.4-99.7% homology with SFTS virus S segment sequences from Chinese and Japanese ticks.