Effects of Dietary Restriction on Spontaneous Dermatitis in NC/Nga Mice
Effects of Dietary Restriction on Spontaneous Dermatitis in NC/Nga Mice
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饮食限制对 NC/Nga 小鼠自发性皮炎的影响
DOI：
10.1177/153537020122601112
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发表时间：
2001
影响因子：
3.2
通讯作者：
H. Takeuchi
中科院分区：
文献类型：
--
作者：
Wenying Fan;K. Kouda;Harunobu Nakamura;H. Takeuchi
在实验动物中，饮食限制延长寿命，改善生理功能，预防或减轻几种疾病的严重程度，包括一些实验性炎症状态。我们研究了饮食限制对自发发生的特应性皮炎（一种炎症性皮肤病）小鼠模型的影响。将NC/Nga小鼠分配至自由进食组或接受另一组所消耗食物量的60%的限制饮食组。根据范围、强度和搔抓时间对皮炎进行表征。然后，我们使用计算机辅助图像分析来量化皮肤切片中的免疫学发现。限制性饮食小鼠的程度、强度评分和抓挠时间比自由进食小鼠逐渐增加。在限制的小鼠中，浸润的炎性细胞（CD 4阳性T细胞、CD 8阳性T细胞、嗜酸性粒细胞和肥大细胞）以及分泌到组织中的白细胞介素-4和-5减少。总之，饮食限制延迟了NC/Nga小鼠自发性皮炎的发作和进展，这种作用可能涉及抑制炎性浸润细胞和细胞因子分泌。
In laboratory animals, dietary restriction prolongs life span, improves physiologic function, and prevents or lessens severity of several diseases including some experimental inflammatory states. We investigated the effect of dietary restriction on a spontaneously occurring mouse model of atopic dermatitis, an inflammatory skin disease. NC/Nga mice were assigned to a group fed ad libitum or to a restricted diet group receiving 60% of the amount of food consumed by the other group. Dermatitis was characterized according to extent, intensity, and scratching time. We then used computer-assisted image analysis to quantify immunologic findings in skin sections. Extent, intensity score, and scratching time in mice with restriction increased more gradually than in mice fed ad libitum. Infiltrating Inflammatory cells (CD4-positive T cells, CD8-positive T cells, eosinophils, and mast cells) as well as interleukin-4 and −5 secreted into tissue were reduced in mice with restriction. In conclusion, dietary restriction delayed onset and progression of spontaneous dermatitis in NC/Nga mice, an effect possibly involving inhibition of inflammatory infiltration cell and cytokine secretion.